Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

In vitro antioxidant activities of total flavonoids extracts from leaves and stems of Adenia lobata (Jacq.) Engl. (Passifloraceae)

Konan Koffi Marcel, Mamyrbékova-Békro Janat Akhanovna*, Békro Yves-Alain, Djié Bi Marc Gabin and Zomi Bi Tra Jérôme
Laboratoire de Chimie Bio Organique et de Substances Naturelles, UFR-SFA,  Université d’Abobo-Adjamé,  02 BP 0801 Abidjan 02, Côte d’Ivoire.  
Email: [email protected]

  •  Accepted: 03 August 2010
  •  Published: 31 January 2011

Abstract

Oxidant stress is implied in numerous illnesses as a triggering factor or partner to their complications. The present work aimed to determine the antioxidant activity of total flavonoids extracts from A. lobata and to identify the molecules that are supposed to be responsible for this activity. The crude extracts, obtained by maceration in 70% (v/v) aqueous methanol, have been extracted successively by hexane, chloroform, ethyl acetate and n-butanol. The crude (S1, S2), ethyl acetate (S1III, S2III) and n-butanol (S1IV, S2IV) extracts underwent a phytochemical screening in order to test the radical scavenging activity against 1, 1’ - diphenyl-2-picrylhydrazyl (DPPH). Qualitative analysis by thin layer chromatography permitted the study to put in evidence the flavonoids content of the different extracts. Contents in phenolic compounds were relatively high in the stems (3604 ± 511, 11 µg GAE/g) in relation to the leaves of A. lobata (3240 ± 711, 05 µg GAE/g). EC50 of 6, 422.5, 430 and 357.5 μg/ml were graphically determined for vitamine C, S1III, S2III, S1IV and S2IVrespectively; fractions S1, S2 and S2IV showed an EC50 greater than 500 μg/ml.

 

Key words: Adenia lobata, flavonoids, antioxidant activity, DPPH, Ivorian pharmacopeia.