Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Pharmacognostic study of male leaves of Trichosanthes dioica Roxb. with special emphasis on microscopic technique

Saurabh Singh1, Lalit Machawal2*and M. G. Chauhan3
1Department of Pharmacognosy, Faculty of Ayurveda Pharmaceutical Sciences, Lovely School of Applied Medical Sciences, Jalandhar, India. 2Amity Institute of Seabuckthorn Research, Amity University, Noida, Uttar Pradesh, India. 3Institute of Post Graduate Teaching and Research in Ayurveda, Gujarat Ayurveda University Jamnagar, Gujarat, India.
Email: [email protected]

  •  Accepted: 08 June 2010
  •  Published: 31 July 2010


Today sophisticated modern research tools for evaluation of the plant drugs are available but microscopic method is one of the simplest and cheapest methods to start with for establishing the correct identity of the source materials. Pharmacognostic investigation of the fresh, powdered and anatomical sections of the leaves of Trichosanthes dioica Roxb was carried out to determine its macro- and microscopical characters and also some of its physical constants. Externally, the leaves possess a cordate base, a sinuate and dentate margin, acute to acuminate apex and both surfaces are very rough with rigid hairs surface. Internally, its shows the presences of an anomocytic stomata, unicellular, both glandular and simple covering trichomes are scattered as such throughout or attached with the cells of the epidermis. Majority of the glandular trichomes are with 4 to 5 celled uniseriate stalk and unicellular head very few are short and with uni- to bicellular stalk and uni- to multicellular head especially from that of petiole region. Simple covering multicellular uniseriate thick walled trichomes are of various sizes and usually of the cells of both simple and glandular trichomes are often embedded with cystolith. Phytochemical studies of the powdered leaves revealed the presences of alkaloids, resins, glycosides, flavonides and some carbohydrates. The pharmacognostic profile of the leaves will assist in standardization for quality, purity and sample identification.


Key words: Trichosanthes dioica, pharmacognostic standardization, male leaf morphology.