In vivo evaluations were made to assess the pesticide activity of lihocin against fresh water fishChanna punctatus and its ultimate mode of action on fish protein metabolism. Biochemical studies show that after exposing the fish to sub lethal dose of lihocin, total protein levels significantly decreased while FAA, glutamine, alkaline phosphatases, acid phosphatases, AlAT, AAT, GDH, AMP deaminase and adenosine deaminase were significantly enhanced in the liver, brain and kidney tissues of C. punctatus. The alterations in all the aforementioned biochemical parameters were significantly (p< 0.05) time and dose dependent. As such, the negative impact of lihocin was shown on the respiratory, as well as, energy production of the fish.
Key words: Channa punctatus, enzyme activity, Lc50, lihocin (OC) pesticidal impact, protein metabolism.
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