Journal of
Toxicology and Environmental Health Sciences

  • Abbreviation: J. Toxicol. Environ. Health Sci.
  • Language: English
  • ISSN: 2006-9820
  • DOI: 10.5897/JTEHS
  • Start Year: 2009
  • Published Articles: 208

Full Length Research Paper

Induction of oxidative stress and apoptosis in human neutrophils by p-phenylenediamine

Zineb Elyoussoufi
  • Zineb Elyoussoufi
  • Department of Biology, Ain Chock Faculty of Sciences, Laboratory of Physiology and Molecular Genetics, Genetics, associated with CNRST, Hassan II University, Casablanca, Morocco
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Khadija Mounaji
  • Khadija Mounaji
  • Department of Biology, Ain Chock Faculty of Sciences, Laboratory of Physiology and Molecular Genetics, Genetics, associated with CNRST, Hassan II University, Casablanca, Morocco
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Rachida Cadi
  • Rachida Cadi
  • Department of Biology, Ain Chock Faculty of Sciences, Laboratory of Physiology and Molecular Genetics, Genetics, associated with CNRST, Hassan II University, Casablanca, Morocco
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Norddine Habti
  • Norddine Habti
  • Laboratory of Hematology, Experimental Medicine and Biotechnology, associated with CRTS, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca, Morocco
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Said Motaouakkil
  • Said Motaouakkil
  • Medical intensive care unit, Ibn Rochd university hospital, Casablanca, Morocco
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  •  Accepted: 13 July 2013
  •  Published: 31 August 2013

Abstract

Exposure to paraphenylenediamine (PPD), a derivative of paranitroaniline widely used as an oxidisable hair dye, is often associated with the development of allergic contact dermatitis. Such reactions involve activation of the subject’s immune system and it is known that neutrophils are the first cells to arrive at the site of perturbation. In view of its pivotal role in the initiation and amplification of inflammation, the fate of the neutrophil in presence of PPD requires attention. Thus, the purpose of the present study was to evaluate the vitro effects of PPD on human neutrophils viability and on reactive oxygen species production. Neutrophils from healthy volunteers were incubated with three concentrations of PPD (11.5, 23 and 46 µM). Apoptosis was evaluated by light microscopy and DNA gel electrophoresis, and oxidative stress was evaluated by spectrophotometry. Results showed that PPD induces neutrophils apoptosis in a dose and time dependent manner with a LC50 value of 23 µM at 6 h of treatment. Furthermore, at 5 h of treatment, PPD markedly increased lipid peroxidation (47%) and enhanced the activity of catalase, glutathione reductase and superoxide dismutase. These results suggest that PPD can induce neutrophils apoptosis and an oxidative stress.

 

Key words: Paraphenylenediamine, neutrophils, apoptosis, oxidative stress, catalase, lipid peroxidation, glutathione reductase, superoxide dismutase.