Journal of
Toxicology and Environmental Health Sciences

  • Abbreviation: J. Toxicol. Environ. Health Sci.
  • Language: English
  • ISSN: 2006-9820
  • DOI: 10.5897/JTEHS
  • Start Year: 2009
  • Published Articles: 208

Full Length Research Paper

Ameliorative effects of melatonin on brain biochemical changes induced by subchronic co-administration of chlorpyrifos and cypermethrin in male Wistar rats

S. B. Idris
  • S. B. Idris
  • Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, Nigeria.
  • Google Scholar
S. F. Ambali
  • S. F. Ambali
  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ilorin, Nigeria.
  • Google Scholar
M. M. Suleiman
  • M. M. Suleiman
  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
  • Google Scholar

  •  Received: 24 November 2016
  •  Accepted: 21 December 2016
  •  Published: 31 January 2017


Ambali SF, Idris SB, Onukak C, Shittu M, Ayo JO (2010b). Ameliorative effect of vitamin C on short term sensorimotor and cognitive changes induced by acute chlorpyrifos exposure in Wistar rats. Toxicol. Ind. Health 26:547-558.


Ambali SF, Makinde AO, Shittu M, Adeniyi SA Mowuogwu F (2012). Alleviating effects of Phyllantus niruri on sensorimotor and cognitive changes induced by sub acute chlopyrifos exposure in wistar rats. Am. J. Med. Med. Sci. 2:50-58.


Bayır A, Kara H, Kıyıcı A, Kıyıcı H, Ak A (2011). The effects of melatonin on oxidative stress markers in an animal model of radiocontrast-induced nephropathy. Biomed. Res. 22(3):311-318.


Belma G, Aylin G, Filiz H (2001). Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol. Toxicol. Lett. 118:139-146.


Draper HH, Hardley M (1990). Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 186:421-431.


Elhalwagy ME, Zaki NI (2009). Comparative study on pesticide mixture of organophosphorus and pyrethroid in commercial formulation. Environ. Toxicol. Pharmacol. 28:219-224


Ellman GC, Courtney KO, Andres V, Featherstone RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7:88-95.


Esparza JL, Gomez M, Rosa NM, Paternain JL, Mallol J, Domingo JL (2005). Melatonin reduces oxidative stress and increases gene expression in the cerebral cortex and cerebellum of aluminum-exposed rats. J Pineal Res 39:129-136.


Freitas RM, Vasconcelos SM, Souza FC, Vianna GS, Fonteles MM (2005). Oxidative stress in the hippocampus pilocarpine induced status epilepticus in Wistar rats. FEBS J. 272:1307-1312.


Garber JC, Barbee RW, Bielitzki JT, Clayton LA, Donovan JC, Hendriksen CF, Kohn DF, Lipman NS, Locke PA, Melcher J, Quimby FW (2011). Guide for the care and use of laboratory animals. The National Academic Press, Washington DC. 8:220.


Gulcin I, Beydemir S, Hisar O, Koksal1 E, Reiter RJ (2009). Melatonin administration increases antioxidant enzymes activities and reduces lipid peroxidation in the rainbow trout (Oncorhynchus mykiss, Walbaum) erythrocytes. Turk. J. Vet. Ani. Sci. 33(3):241-245.


Gulterkin FN, Patat S, Akca H, Altunas I (2006) Melatonin can suppress the cytotoxic effects of chlopyrifos of human HepG2 cell lines. Human Exp. Toxicol. 25(2):47-55.


Krishnanamoorthy GP, Ventaraman A, Arunkumar RC, Vignesh MM, Arunakaran J (2007). Ameliorative effect of vitamins α-tocopherol and ascorbic acids) on PCB (Aroclor 1254)-induced oxidative stress in rat epidydymal sperm. Reprod. Toxicol. 23:239-245


Latuszynska J, Luty S, Raszewski G, Tokarska-rodak M, Przebirowska D, Przylepa E, Haratym–maj A (2001). Neurotoxic effect of dermally-applied chlorpyrifos and cypermethrin in Wistar rats. Ann Agric. Environ. Med. 8:163-178


Lowry OH, Rosenbrough NJ, Fair AL, Randall RJ (1952). Protein measurement with folin-phenol reagent . J. Biol. Chem. 193:265-275.


Mauriz JL, Molpeceres V, Garcia-Mediavilla MV, Gonzales P, Barrio JP, Gonzales-Galego J (2007). Melatonin prevents oxidative stress and changes in antioxidant enzyme expression and activity in liver of ageing rats. J Pineal Res. 42(3):222-230.


Raina R, Verma PK, Pankaj NK, Vinay K (2009). Ameliorative effects of α-tocopherol on cypermethrin induced oxidative stress and lipid peroxidation in Wistar rats. Int. J. Med. Med Sci. 1(9):396-399


Reiter RJ, Tan DX, Rossales-Corales S, Manchester LC (2013). The universal nature, unequal distribution and antioxidant nature of melatonin and its derivatives. Mini Rev. Med. Chem. 13(3): 373-384.


Satinderpal S, Shamsherjit K, RamjiDB (2013). Chlopyrifos-induced oxidative stress in rats brain and protective effective effect of grape seed extract. J. Phytopharm. 2(3):26-33


Sekar BM, Jayaraman P, Aarthy P (2010). Screening of acetylcholinesterase in blood and plasma and brain of Wistar rats by NEURELLA D (combination pesticide). Int. J. Pharm. Biol. Sci. 1(2):574-578.


Tohru F, Masuko U (2011). Superoxide dismutases:Role in redox signalling, vascular function and diseases. Antioxidants and redox signalling 15:1583-1606.


Tsakiris S, Angelogianni P, Schulpis KH, Stavridis JC (2000). Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals. Clin. Biochem. 33:103-106


Uchendu C, Ambali SF, Ayo JO, lasisi IO, Umosen AJ (2013). Subacute chlopyrifos –induced alterations in serum lipids and some oxidative stress biomarkers in male Wistars: beneficial effect of acetyl- L-carnitine. Toxicol. Environ. Chem. 95(3):483-494.


United States Environmental Protection Agency (USEPA) (2000). Chlorpyrifos: HED Preliminary Risk Assessment for the Reregistration Eligibility Decision (RED) document. Chemical No. 059101. Barcode: D260163, Case: 818975, Submission: S568580


Wielgomas B, Krechniak J (2007). Effect of α-cypermethrin and chlorpyrifos in a 28‑day study on free radical parameters and cholinesterase activity in Wistar rats. Pol. J. Environ. Stud. 16:91-95.