Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 350

Full Length Research Paper

Comparison of fluorescence polarization assay and enzyme-linked immunosorbent assay for the diagnosis of bovine paratuberculosis

Raquel Torres-Velez
  • Raquel Torres-Velez
  • CENID Microbiología INIFAP: Carretera México Toluca Km 15.5, Colonia Palo Alto, Cuajimalpa, C.P. 05110, CDMX, México.
  • Google Scholar
Marco Antonio Santillan-Flores
  • Marco Antonio Santillan-Flores
  • CENID Microbiología INIFAP: Carretera México Toluca Km 15.5, Colonia Palo Alto, Cuajimalpa, C.P. 05110, CDMX, México.
  • Google Scholar
Dionisio Cordova-Lopez
  • Dionisio Cordova-Lopez
  • CENID Microbiología INIFAP: Carretera México Toluca Km 15.5, Colonia Palo Alto, Cuajimalpa, C.P. 05110, CDMX, México.
  • Google Scholar
Olga Lidia Martinez-Martinez
  • Olga Lidia Martinez-Martinez
  • Universidad del Valle de México CAMPUS Coyoacán: Calzada de Tlalpan 3016, Ex hacienda de Coapa, C. P. 04910, Delegación Coyoacán CDMX, México.
  • Google Scholar
Claudia Celic Guzmán-Ruiz
  • Claudia Celic Guzmán-Ruiz
  • Universidad de Guanajuato, División Ciencias de la Vida, Campus Irapuato –Salamanca. Ex hacienda, El Copal Km 9, Carretera Irapuato-Silao, CP 36820. Irapuato, Guanajuato, México.
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  •  Received: 11 June 2019
  •  Accepted: 08 August 2019
  •  Published: 31 August 2019

Abstract

Mycobacterium avium subspecies paratuberculosis (Map), is the etiological agent of paratuberculosis, a disease that affects cattle and causes economic losses to the animal husbandry industry. Its opportune diagnosis, in herds, is part of the control measures of the disease; therefore, the objective of this work was to compare paratuberculosis detection of infected bovines with the Fluorescence Polarization Assay (FPA) and Enzyme-linked Immunosorbent Assay (ELISA). Six-hundred and three sera and feces samples, from bovines older than 2 years old were studied. The sera were assessed with the FPA technique using as antigen a protein fraction of 35 kDa obtained from the raw extract of the Map strain 3065, and for ELISA the protoplasmic antigen of the same strain was used. DNA was obtained from the feces and assessed by nested PCR. The correlation of results was established by Kappa Test.  The FPA test had sensitivity (Se) of 88.50% and specificity (Sp) of 91.42% (p ≤0.000); for ELISA Se 83.86% and Sp 89.87% (p ≤0.000) were obtained. Concordance (K) between tests was 0.6742%, and when compared with nested polymerase chain reaction (PCR), the FPA test had K = 0.7314%, while for ELISA it was 0.5771%. The FPA technique using as antigen the protein fraction of 35 kDa showed a higher sensitivity and specificity, moreover it was a simple technique for the determination of the antigen-antibody interaction, and therefore it becomes an alternative diagnostic tool to detect paratuberculosis infected bovines.

Key words: Paratuberculosis, diagnosis, fluorescence polarization assay (FPA), enzime-linked immunoabsorbent assay (ELISA).