Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 347

Full Length Research Paper

Genetic evolution of infectious bursal disease virus in Senegal

Alkaly Badji
  • Alkaly Badji
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
  • Google Scholar
Alpha Amadou Diallo
  • Alpha Amadou Diallo
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
  • Google Scholar
Mariette Ducatez
  • Mariette Ducatez
  • INRA and Université de Toulouse, INP, ENVT UMR 1225 IHAP INRA et ENVT, 23 chemin des Capelles 31076 Toulouse France.
  • Google Scholar
Fatou Tall Lo
  • Fatou Tall Lo
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
  • Google Scholar
Mbaye Mbengue
  • Mbaye Mbengue
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
  • Google Scholar
Moussa Diouf
  • Moussa Diouf
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
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Yacine Samb
  • Yacine Samb
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
  • Google Scholar
Mariame Diop
  • Mariame Diop
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
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Modou Moustapha Lo
  • Modou Moustapha Lo
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
  • Google Scholar
Yaya Thiongane
  • Yaya Thiongane
  • ISRA/LNERV, Laboratoire National de l’Elevage et de Recherches Vétérinaires, BP 2057 Dakar-Hann, Sénégal.
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Jean Luc Guerin
  • Jean Luc Guerin
  • INRA and Université de Toulouse, INP, ENVT UMR 1225 IHAP INRA et ENVT, 23 chemin des Capelles 31076 Toulouse France.
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Rianatou Bada Alambedji
  • Rianatou Bada Alambedji
  • EISMV, Ecole Inter-Etats des Sciences et Médecine Vétérinaires, BP 5027 Dakar-Fann, Sénégal.
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  •  Received: 08 September 2016
  •  Accepted: 20 October 2016
  •  Published: 31 December 2016

Abstract

In recent years very virulent (VV) IBDV strains and classical (CV) IBDV strains re-emerged and caused devastating outbreaks in different parts of the world. In this study, genetic evolution of fifteen IBDVs collected in Senegal in 1979, 1999, 2007, 2012, 2013 and 2014 was characterized to gain information for a better control of IBD. Following RT-PCR, nucleotide sequence of the VP2 hypervariable region was determined and compared with sequences available in GenBank. Phylogenetic analysis showed that the viruses diverged into two genotypes: Very virulent (VV) IBDV and classical virulent (CV) IBDV. The Senegalese field strains of the first genotype (VV) IBDV had 98.9 to 100% identity among themselves, whereas their identity with reported Nigerian (VV) IBDVs ranged between 96.7 and 99%. The close phylogenetic relationship of the Senegalese and Nigerian strains suggests that they likely derived from a common ancestor. In the phylogenetic tree, all the Senegalese (VV) IBDV strains belonged to the African very virulent types (VV2). The genotyping of senegalese field IBDV strains indicated that the majority of viruses circulating in Senegal are (VV) IBDVs and highlights a genetic stability. 
 
Key words: Infectious bursal disease, phylogenetic analysis, Senegal.