Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Synthesis, physical properties, antimicrobial potentials of some mixed antibiotics complexed with transition metals and their effects on alkaline phosphatase activities of selected rat tissues

Ogunniran K. O.1*, Ajanaku K. O.1, James O. O.1, Ajani O. O.1, Nwinyi, C. O.2,  Omonhimin C. A.2 and Allensela M. A.3
  1Department of Chemistry, College of Science and Technology, Covenant University, P. M. B. 1023, Ota, Ogun State. Nigeria. 2Department of Biological Sciences, College of Science and Technology, Covenant University, P. M. B. 1023, Ota, Ogun State. Nigeria. 3Chemistry Department, College of Education, Benin. P. M. B 1144, Benin City. Edo State, Nigeria.
Email: [email protected]

  •  Accepted: 25 July 2008
  •  Published: 31 August 2008

Abstract

 

Mixed ligand metal complexes of chloramphenicol and oxytetracycline were prepared by using Ni(II), Co(II) and Fe(III) metal chloride hexahydrate. They were characterized based on some physical technique and spectroscopic analysis such as AAS, UV, and IR spectroscopy. Based on the analytical and spectroscopic data, the complexes were proposed to have the formulae [ML1L2](Cl)n (where M = Ni(II), Co(II), Fe(III); L1 = chloramphenicol, L2 = oxytetracycline  and n = 2 - 3). IR spectra suggested that both L1 and L2 coordinated to the metal ions in a terdentate manner with ν(O-H), ν(C=O) and ν(N-H) as donor sites in each of the ligands. The complexes were proposed to be of octahedral. The synthesized complexes, in compares to their ligands, were screened for their antibacterial activity against isolated strains of Escherichia coliStaphylococcus aureus and Klebsiella pneumonia by using diffusion method. The activity data showed the metal complexes to be more potent antibacterial than the parent drugs against the three species. Toxicology tests against some tissues of albino rat (Rattus novergicuss) revealed toxicity of the complexes as compared to the parent drugs. Ni(II) complex was found to possess no significant difference (P > 0.05) in alkaline phosphatase from both homogenates of liver and kidney and rat serum. However, Co(II) and Fe(III)  complexes were found to significantly increase (P < 0.05) alkaline phosphatase from homogenates of liver and kidney tissues of the tested doses but there was no significant difference (P > 0.05) in alkaline phosphatase from rat serum.

 

Key words: Metal complexes, complexation, antibiotics, antimicrobial properties, alkaline phosphatase.