Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2768

Full Length Research Paper

Estimation of the nuclear DNA content in some representative of genus Dioscorea

J. E. Obidiegwu1, 2, 3*, E. Rodriguez4, E. E. Ene-obong3, J. Loureiro4, C. O. Muoneke3, C. Santos4, M. Kolesnikova-Allen5 and R. Asiedu1
1International Institute of Tropical Agriculture, C/O L. W. Lambourn and Co., Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, UK. 2National Root Crops Research Institute, Umudike, PMB 7006, Umuahia, Abia State, Nigeria. 3Department of Agronomy, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria. 4CESAM and Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810 - 193 Aveiro, Portugal. 5Biotechnology Unit, Tun Abdul Razak Research Centre Brickendonbury Hertford, UK SG13 8NL.
Email: [email protected]

  •  Accepted: 20 April 2009
  •  Published: 31 May 2009

Abstract

Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cultivar and considered specie. Estimated nuclear DNA sizes ranged from 0.702 ± 0.004 pg for G1 nuclei of diploid Dioscorea dumetorum to 2.573 ± 0.020 pg for G1 nuclei of octoploid Dioscorea cayenensis. Our result showed that there is a decrease in genome size with higher ploidy level. The study contributes a step towards a genome understanding of this group of plants.

 

Key words: Dioscorea, DNA content, flow cytometry, genome, ploidy level.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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