Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2758

Full Length Research Paper

Improved production of β-galactosidase and β-fructofuranosidase by fungi using alternative carbon sources

Diandra de Andrades
  • Diandra de Andrades
  • Centro de Ciências Biológicas e da Saúde ? UNIOESTE - Rua: Universitária 2069 CEP:85814-110, Cascavel, Paraná, Brazil.
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Vanessa Cristina Arfelli
  • Vanessa Cristina Arfelli
  • Centro de Ciências Biológicas e da Saúde ? UNIOESTE - Rua: Universitária 2069 CEP:85814-110, Cascavel, Paraná, Brazil.
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Alesandra Oriente
  • Alesandra Oriente
  • Centro de Ciências Biológicas e da Saúde ? UNIOESTE - Rua: Universitária 2069 CEP:85814-110, Cascavel, Paraná, Brazil.
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Caroline Henn
  • Caroline Henn
  • Divisão de Reservatório ? MARR.CD, Itaipu Binacional, Avenida Tancredo Neves, 6731, CEP 85866-900 ? Foz do Iguaçu, Paraná, Brazil.
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Veridiana Araujo Alves da Costa Pereira
  • Veridiana Araujo Alves da Costa Pereira
  • Divisão de Reservatório ? MARR.CD, Itaipu Binacional, Avenida Tancredo Neves, 6731, CEP 85866-900 ? Foz do Iguaçu, Paraná, Brazil.
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Rita de Cássia Garcia Simão
  • Rita de Cássia Garcia Simão
  • Centro de Ciências Médicas e Farmacêuticas ? UNIOESTE - Rua: Universitária 2069 CEP:85814-110, Cascavel, Paraná, Brazil.
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Jose Luis da Conceição Silva
  • Jose Luis da Conceição Silva
  • Centro de Ciências Médicas e Farmacêuticas ? UNIOESTE - Rua: Universitária 2069 CEP:85814-110, Cascavel, Paraná, Brazil.
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Marina Kimiko Kadowaki
  • Marina Kimiko Kadowaki
  • Centro de Ciências Médicas e Farmacêuticas ? UNIOESTE - Rua: Universitária 2069 CEP:85814-110, Cascavel, Paraná, Brazil.
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  •  Received: 04 August 2014
  •  Accepted: 19 March 2015
  •  Published: 30 March 2015

Abstract

The influence of alternative carbon sources as inducers of -galactosidase and -fructofuranosidase by filamentous fungi (that are, Aspergillus aculeatus, Chrysonilia sitophila, Gliocladium virens, Aspergillus fumigatus and Trichoderma longibrachiatum), recently isolated from Brazil’s Atlantic Forest biome has been investigated. The greatest levels of intracellular β-galactosidase activity were obtained using orange peel waste (56.31 U/mL) with A. aculeatus, rice straw (22.57 U/mL) with G. virens, sorghum straw (16.48 U/mL) with C. sitophila, and passion fruit peel with either A. fumigatus (17.26 U/mL) or T. longibrachiatum (17.53 U/mL). The most effective intracellular -fructofuranosidase activity was obtained by A. aculeatus using trub (409.46 U/mL) or passion fruit peel (44.59 U/mL). Thus, alternative carbon sources, such as orange peel and trub, exhibit great potential as inducers for the production of these enzymes. Such fungal isolates from the Atlantic Forest of Paraná, Brazil are promising candidates for generating significant amounts of β-galactosidase and -fructofuranosidase using abundant and inexpensive agro-industrial substrates. 
 
Key words: Agro-industrial residue, Atlantic Forest, lactase, invertase, fungus.