Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2753

Full Length Research Paper

Isolation and characterization of angiotensin I-converting enzyme inhibitor peptides derived from porcine hemoglobin

Yan Ren1,2, De-Guang Wan1,2, Xian-ming Lu1,2, Lu Chen1,2, Tian-e Zhang3 and Jin-Lin Guo1,2*
1State Key Laboratory of Systems Research and Development of TCM Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People’s Republic of China. 2Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People’s Republic of China. 3Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People’s Republic of China.
Email: [email protected]

  •  Accepted: 15 August 2011
  •  Published: 09 December 2011

Abstract

In China, porcine blood has a long history as a food with medicinal effects and for treating strokes, an obvious clinical consequence of hypertension. However, the active ingredients are still unknown and need to be clarified. This study described the isolation, characterization and animal experiments of angiotensin converting enzyme (ACE)-inhibitor peptides derived from porcine blood. The highly active low-molecular-weight hydrolysates were obtained from pepsin digestion of discolored porcine blood. After isolation of the hydrolysate, an active fraction containing three peptides was obtained. These peptides were analyzed by MALDI-TOF-MS. They were WVPSV (P1), YTVF (P2), VVYPW (P3), with median inhibitory concentrations of 0.368, 0.226, 0.254 mg/ml, respectively. They were first found in porcine blood. Additionally, a digestion test was performed to verify the antihypertensive effect of the peptides in vitro. After digestion with gastrointestinal proteases, the ACE-inhibitor activity of these peptides was enhanced. When these peptides were administered orally to spontaneously hypertensive rats at a dose of 10 mg/kg, a temporary antihypertensive activity was observed at 3 and 15 h after administration. These findings suggested that the peptides in porcine blood might have potential as antihypertensive agents.

 

Key words: Angiotensin I-converting enzyme, inhibitor activity, peptide, porcine hemoglobin.