Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2740

Full Length Research Paper

High yield generation of hepatocyte like cells from adipose derived stem cells

Abbas Sahebghadam Lotfi1*, Alireza Khoshdel1, Masoud Soleimani2, Morteza Daliri3,Vahid Razban3 and Behzad Adibi1
  1Departments of Clinical Biochemistry,  Faculty of Medical Sciences, Tarbiat Modares University, P. O. Box 14115-111, Tehran, Iran. 2Departments of Hematology, Faculty of Medical Science, Tarbiat Modares University, P. O. Box 14155- 6343,  Tehran, Iran. 3National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Email: [email protected], [email protected]

  •  Accepted: 22 November 2011
  •  Published: 16 March 2012

Abstract

 

Mesenchyma stem cells (MSCs) are multipotent cells. They are capable of transforming into several mesoderm lineages. They can be separated from different tissues, such as bone marrow, umbilical cord blood, peripheral blood, and adipose tissue. Stem cell based therapies for the repair and regeneration of individual tissues and organs suggest a paradigm shift which may supply optional therapeutic solutions for a number of diseases. Embryonic and induced pluripotent stem cells have hypothetically been beneficial, although there are various restrictions. Bone marrow-derived mesenchyma stem cells when compared to adipose-derived stem cells (ADSCs), they were obviously capable of differentiating into cells and tissues with mesodermal origin. Adipose tissue is prevalent and easily available in large quantities. The intent of this investigation was the isolation of mesenchymal stem cells from rat adipose tissue, the potential of MSCs in differentiating into osteogenic and adipogenic lineages, describe the ADSCs surface expression of the classic markers by using flow cytometric analysis, and their transformation to hepatocyte like cells. Immunocytochemical analysis displayed that ADSCs express albumin and α-fetoprotein during differentiation.  Knowledge of these cells may also demonstrate many benefits in cell-based therapies for tissue repair, regeneration, or tissue engineering.

 

Key words: Hepatocyte-like cells, mesenchymal stem cells, cell therapy and adipose derived stem cell.