Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2740

Full Length Research Paper

Effects of various medium compositions and wounding treatments on in vitro growth and regeneration of bird of paradise(Strelitzia reginae)

J. J. North1, P. A. Ndakidemi2* and C. P. Laubscher
  1Faculty of Applied Sciences, Cape Peninsula University of Technology, P.O. Box 652, Cape Town 8000, South Africa. 2The Nelson Mandela African Institute of Science and Technology, P. O. Box 447-Arusha-Tanzania.
Email: [email protected]

  •  Accepted: 16 February 2012
  •  Published: 16 March 2012

Abstract

 

The aim of this study was to investigate the use of antioxidants, wounding treatments and hormone concentrations in efforts to overcome phenolic oxidation and stimulate axillary bud proliferation. Significant results were achieved with 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) concentrations on explants discoloration and callus formation. The antioxidant treatments, activated charcoal (AC) and ascorbic acid (AA) significantly affected explant discoloration, the induction of callus and the length of roots developed. Wounding treatments resulted in a reduction of plant height, an increase in both explants discoloration and callus formation. The most effective treatment in reducing explants discoloration at the media contact point was achieved in interactive effects of higher NAA and BAP concentrations (0.1 mg.l-1 NAA; 3 mg.l-1 BAP and 0.5 mg.l-1NAA; 5 mg.l-1 BAP) without wounding. Interactions between antioxidants and wounding treatments resulted in the absolute absence of callus induction in all treatments involving ascorbic acid.

 

Key words: Ascorbic acid, activated charcoal, 6-benzylaminopurine (BAP), callus formation, discoloration, 1-naphthalene acetic acid (NAA).

Abbreviation

MS, Murashige and Skoog (1962); AC, activated charcoal; AA, ascorbic acid; BAP, 6-benzylaminopurine; NAA, 1-naphthalene acetic acid.