300 urine samples with significant bacteriuria collected from 3 hospitals in Mubi were analysed for presence of Enterobacteriaceae bacteria. 187 urine samples comprising 68.9% female and 31.1% male yielded Enterobacteriaceae bacteria growth. The isolates include Escherichia coli 51.5%, Klebsiella pneumonia 24.4%, Klebsiellaoxytoca 3.1%, Enterobacter aerogenes 9.7% and Citrobacter freundii 10.9%. Antibiotic resistance profile obtained revealed high resistance of isolates to ampicillin 37.5%, ciprofloxacin 36.4% and co-amoxyclav 21.3%. Streptomycin, nalidixic acid, cephalexin and gentamicin highly inhibited growth of the organisms tested. Gentamicin resistance rate of 17% was obtained while curing of selected donor isolates showed that gentamicin resistance in 75.8% of the isolates were plasmid mediated or located on mobile genetic element. Transfer rates of 34.8% and 41.1% respectively were obtained for inter-generic and intra-species transfer of gentamicin resistance genes (Gmr) among the Enterobacteriaceae isolates. Evidence of transferability of Gmr invitro concurs to the assertion that under favorable conditions conjugal transfer of gentamicin resistance determinants and hence R plasmid could occur in vivo.
Key words: Enterobacteriaceae, gene transfer, gentamicin resistance genes, plasmid, curing, conjugation.
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