African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Bulb and vegetative characteristics of garlic (Allium sativum L.) from in vitro culture through acclimatization and field production

  E. I. Metwally1, M. E. El-Denary2, A. M. K. Omar3, Y. Naidoo4 and Y. H. Dewir1*        
  1Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt. 2Potatoes and Vegetables Department, Horticulture Research Institute, Giza, Egypt. 3Department of Horticulture, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida 919, Libya. 4School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000,South Africa.
Email: [email protected]

  •  Accepted: 05 October 2012
  •  Published: 30 November 2012



This study reports the field performance of tissue-cultured garlic plants (cultivar Balady) upon their acclimatization for four successive generations. Bulb weight reached 0.7, 3.4, 62.1 and 87.9 g at the first, second, third and fourth vegetative generations, respectively. The bulb was small and non-divided in the first vegetative generation. The number of cloves per bulb was 2.4, 45.8 and 54.0 at the second, third and fourth vegetative generations, respectively. Bulb weight and cloves number in the original cultivar were 76.4 g and 55.2 cloves per bulb, respectively. The bulb development rate was high for three vegetative generations. However, it was the closest between the third and the fourth vegetative generation in which garlic plants reached a comparable size of the original cultivar. This study indicates that garlic plantlets derived throughtissue culture takes four vegetative generations (four years) to reach the commercial size. These developed plantlets are considered as a new source for breeding and improvement of garlic crop.


Key words: Acclimatization, bulbs, cultivation, garlic, tissue culture.