African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Trichoderma-bean interaction: Defense enzymes activity and endophytism

Omari Dangelo Forlin Dildey
  • Omari Dangelo Forlin Dildey
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Laline Broetto
  • Laline Broetto
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Bruna Broti Rissato
  • Bruna Broti Rissato
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Edilaine Della Valentina Gonçalves-Trevisoli
  • Edilaine Della Valentina Gonçalves-Trevisoli
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Sidiane Coltro-Roncato
  • Sidiane Coltro-Roncato
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Emanuele Guandalin Dal’Maso
  • Emanuele Guandalin Dal’Maso
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Cristiane Claudia Meinerz
  • Cristiane Claudia Meinerz
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Nicanor Pilarski Henkemeier
  • Nicanor Pilarski Henkemeier
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Jose Renato Stangarlin
  • Jose Renato Stangarlin
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Odair Jose Kuhn
  • Odair Jose Kuhn
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar
Tulya Fernanda Barrientos Webler
  • Tulya Fernanda Barrientos Webler
  • State University of West Paraná - UNIOESTE, Marechal Cândido Rondon, Paraná, Brazil.
  • Google Scholar


  •  Received: 08 September 2016
  •  Accepted: 28 September 2016
  •  Published: 27 October 2016

Abstract

Biotic inducing agents such as Trichoderma spp. are an alternative for the induction of resistance by activating defense mechanisms of plants. This work aimed to study the interaction between bean and Trichoderma spp. evaluating the effects of the induction of resistance in bean through the defense enzymes activity and ability to endofitism. The experimental was design in randomized blocks, with four replications, being 21 isolates of Trichoderma spp. and control. The isolates were inoculated at planting, with adding 0.8 ml of the suspension 1×108 conidia/ml in groove, in sterilized soil. After 30 days, leaf and roots samples were taken to determine the enzyme activity. To verify the endophytic colonization capacity of isolated, bean root fragments were sanitized and placed in Petri dishes containing PDA medium. Data were submitted to analyzes of variance and compared by Scott-Knott test (p<0.05). The enzymatic activity of peroxidase, polyphenoloxidase and phenylalanine ammonia-lyase was not significant, but the β-1-3-glucanase activity in leaf tissue was suppressed by isolates TI1, TM1, TLB15 (Trichoderma virens), TI2, TLB3, TLB4, TLB12, TOD1, TOD3 (Trichoderma harzianum), TLB6 (Trichoderma asperellun), TLB14, TLB17 (Trichoderma koningiopsis) and TOD2B (Trichoderma longibrachiatum). The isolates TM4, TLB9, TLB15 (T. virens), TI2, TI4, TLB2, TOD1 (T. harzianum) and TLB17 (T. koningiopsis), were able to colonize endophytically the bean roots. Trichoderma has endophytic capacity and interfere in β-1-3-glucanase activity.

 

Key words: Induced resistance, Phaseolus vulgaris L., Trichoderma spp.