African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Physiological quality of hybrid maize seeds through respiratory and enzymatic activities

Heloisa Oliveira dos Santos
  • Heloisa Oliveira dos Santos
  • Agronomy/Crop Science, University of Lavras-UFLA/DAG, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil.
  • Google Scholar
Iolanda Vilela Von Pinho
  • Iolanda Vilela Von Pinho
  • Biology /Genetics and Plant Breeding, University of Lavras-UFLA/DBI, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil.
  • Google Scholar
Édila Vilela de Resende Von Pinho
  • Édila Vilela de Resende Von Pinho
  • Agronomy/Crop Science, University of Lavras-UFLA/DAG, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil.
  • Google Scholar
Raquel Maria de Oliveira Pires
  • Raquel Maria de Oliveira Pires
  • Agronomy/Crop Science, University of Lavras-UFLA/DAG, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil,
  • Google Scholar
Valkíria Fabiana da Silva
  • Valkíria Fabiana da Silva
  • Agronomy/Crop Science, University of Lavras-UFLA/DAG, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil.
  • Google Scholar
Maria Laene Moreira de Carvalho
  • Maria Laene Moreira de Carvalho
  • Agronomy/Crop Science, University of Lavras-UFLA/DAG, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil.
  • Google Scholar
Roseane Maria Evangelista Oliveira
  • Roseane Maria Evangelista Oliveira
  • Agronomy/Crop Science, University of Lavras-UFLA/DAG, Câmpus Universitário, Caixa Postal 3037, CEP 37200-000, Lavras/MG, Brazil.
  • Google Scholar


  •  Received: 28 September 2015
  •  Accepted: 17 February 2016
  •  Published: 19 May 2016

Abstract

With the increased demand for high quality maize seeds, the market absorbs innovations that add speed and reliability in the tests that enable differentiation of potential performance of seed lots. This study aims to evaluate the combination of the physiological tests with the expression of enzymes of respiratory route for differences in vigor levels in lots of hybrid seed corn. The percentage of germination, germination first count, emergence on tray, index of emergency speed, accelerated aging and respiration rate were evaluated by Pettenkoffer and Titulation method. Furthermore, the expression of alpha amylase, alcohol dehydrogenase, malate dehydrogenase and pyruvate decarboxylase was analyzed. With the results, it is determined that the vigor tests analyzed, together with the tests of enzymatic expressions of respiratory route were efficient to detect different levels of force in hybrid seed corn. Lot 1 had higher physiological quality while lot 2 had the lowest vigor compared to the other lots. With the vigor tests and expression of enzymes, it was possible to differentiate the quality of the seeds and therefore it can be recommended as reliable tests for defining the quality of seed lots.

Key words: Respiration, enzyme expression, vigor, viability.