African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Plant extracts enhancers of defense response in ponkan mandarin Seedlings against Alternaria alternate f. spp. citri infection

Kedma Maria Silva Pinto
  • Kedma Maria Silva Pinto
  • Universidade Federal Rural de Pernambuco, Unidade Acadêmica de Garanhuns – PE, Brazil.
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Paulo Alexandre Fernandes Rodrigues de Melo
  • Paulo Alexandre Fernandes Rodrigues de Melo
  • Universidade Estadual do Maranhão, São Luis – MA, Brazil.
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Janaína Marques Mondego
  • Janaína Marques Mondego
  • Universidade Estadual do Maranhão, São Luis – MA, Brazil.
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Luciana Cordeiro do Nascimento
  • Luciana Cordeiro do Nascimento
  • Universidade Federal da Paraíba, Campus Areia, Brazil.
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Maria Izabel Mendes Marques Cortez
  • Maria Izabel Mendes Marques Cortez
  • Universidade de Trás os Montes e Alto Douro / Centre for the Research and Technology of Agro-Environmental and Biological Sciences, Vila Real Portugal.
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Alfredo Augusto de Carvalho Aires
  • Alfredo Augusto de Carvalho Aires
  • Universidade de Trás os Montes e Alto Douro / Centre for the Research and Technology of Agro-Environmental and Biological Sciences, Vila Real Portugal.
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Antonio Pereira dos Anjos Neto
  • Antonio Pereira dos Anjos Neto
  • Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, São Paulo, Brazil.
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Robson Luís Silva de Medeiros
  • Robson Luís Silva de Medeiros
  • Faculdade de Ciências Agrárias e Veterinárias - Universidade Estadual Paulista, Jaboticabal – SP., Brazil.
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Jose Ribamar Gusmao Araujo
  • Jose Ribamar Gusmao Araujo
  • Universidade Estadual do Maranhão, São Luis – MA, Brazil.
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Hilderlande Florencio da Silva
  • Hilderlande Florencio da Silva
  • Universidade Federal da Paraíba, Campus Areia, Brazil.
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  •  Received: 29 January 2018
  •  Accepted: 15 February 2018
  •  Published: 29 March 2018

Abstract

The objective of this study was to determine the elicitor potential of plant extracts from Caatinga biome by enzyme activity and epidemiological components of Alternaria brown spot (Alternaria alternate f. spp. citri). These were prepared ethanolic, dichloromethanic, and aqueous extracts from 14 plants (Anadenanthera macrocarpa, Schinopsis brasiliensis, Maytenus rigida, Caesalpinia pyramidalis, C. ferrea, Peltophorum dubium, Capariscyn ophallophora, Ziziphus joazeiro, Mimosa hostilis, Momordica charantia, Erythrina velutina, Cleome hassleriana, Sideroxylum obtusifolium Spondias tuberosa) after dilution, (1mg/mL) they were sprayed on Citrus reticulate ‘Ponkan’seedlings and after 0, 24, 48, 72 and 96 h the leaves were collected for determination of polyphenol oxidase and peroxidase activity. For epidemiological disease components, leaves were collected 72 h after spraying, deposited on Petri dishes with moist paper, inoculated with a spore suspension of A. alternata f. spp. citri (2x105 conidia mL-1) and evaluated for 12 days initial and final severity, area below the progress curve of disease and protection. The extracts of A. macrocarpa and M. rigida potentiated the polyphenol oxidase and peroxidase activity which had great potential in the management of the disease, reducing the final severity and area under the disease progress curve, providing high levels of control.

Key words: Citrus reticulata, alternaria brown spot, elicitor.