Abstract

Among bio-chemical techniques, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is most widely used due to its validity and simplicity for describing genetic structures of group of plants. In the present study, SDS-PAGE was used to confirm sunflower hybrids. Four male sterile lines, four restorer lines and their sixteen F₁ hybrids were analyzed. Based on results of electrophoretic band spectra, Jaccard’s similarity index (S) was calculated for all possible pairs. The similarity matrix, thus, generated was converted to a dissimilarity matrix and used to construct dendrogram, using unweighed pair-group method with arithmetic means. Euclidean distance of 1.0 was observed among 5 comparisons, whereas maximum distance (3.87) was observed for 1 comparison only. In most of the cases, female parents, along with their respective crosses, were found in one cluster, indicating that SDS-PAGE can be used reliably for the identification of hybrids in sunflower.

Key words: Sunflower, sodium dodecyle sulphate polyacrylamide gel electrophoresis, F₁ hybrids, heterosis.

Abbreviation

SDS-PAGE, Sodium dodecyl sulphate-polyacrylamide gel electrophoresis; S, Jaccard’s similarity index; CMS, cytoplasmic male sterile.