African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Confirmation of sunflower F1 hybrids using SDS-PAGE analysis

Sher Aslam Khan1, Habib Ahmed2*, Shah Masaud Khan1, Ayub Khan1, Sardar Ali1 and Muhammad Saeed1
1Department of Agriculture, Hazara University, Haripur, Pakistan. 2Department of Genetics, Hazara University, Mansehra, Pakistan.
Email: [email protected]

  •  Accepted: 25 May 2010
  •  Published: 31 July 2010

Abstract

Among bio-chemical techniques, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is most widely used due to its validity and simplicity for describing genetic structures of group of plants. In the present study, SDS-PAGE was used to confirm sunflower hybrids. Four male sterile lines, four restorer lines and their sixteen F1 hybrids were analyzed. Based on results of electrophoretic band spectra, Jaccard’s similarity index (S) was calculated for all possible pairs. The similarity matrix, thus, generated was converted to a dissimilarity matrix and used to construct dendrogram, using unweighed pair-group method with arithmetic means. Euclidean distance of 1.0 was observed among 5 comparisons, whereas maximum distance (3.87) was observed for 1 comparison only. In most of the cases, female parents, along with their respective crosses, were found in one cluster, indicating that SDS-PAGE can be used reliably for the identification of hybrids in sunflower.

 

Key words: Sunflower, sodium dodecyle sulphate polyacrylamide gel electrophoresis, F1 hybrids, heterosis.

Abbreviation

SDS-PAGE, Sodium dodecyl sulphate-polyacrylamide gel electrophoresis; S, Jaccard’s similarity index; CMS, cytoplasmic male sterile.