African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

The ameliorative role of cranberry extract and bone marrow cells against chlorambucil cytotoxicity in rat fertility

Ebtesam H. O. Nafie*
  • Ebtesam H. O. Nafie*
  • Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt.
  • Google Scholar
Eman Khater
  • Eman Khater
  • Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt.
  • Google Scholar
Mohamed Awwad
  • Mohamed Awwad
  • Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt.
  • Google Scholar
Mohamed Zowail
  • Mohamed Zowail
  • Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt.
  • Google Scholar
Kholud Hegazy
  • Kholud Hegazy
  • Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt.
  • Google Scholar


  •  Received: 14 December 2016
  •  Accepted: 23 January 2017
  •  Published: 08 February 2017

Abstract

The objective of the current study was to evaluate and compare the effectiveness of cranberry extracts and bone marrow cells against chlorambucil (CHB) effect on rats' fertility. Forty adult male albino rats were divided randomly into eight equal groups as the following; normal control, rats injected orally with 0.2 mg/kg of CHB for 14 days, rats injected orally with 100 mg/kg of cranberry extract (CB) for ten days, rats intravenously injected with bone marrow cells (BMC) through tail vain, rats protected with both CB and BMC, rats treated with CHB+CB, rats treated with CHB+BMC and rats treated with CHB+BMC+CB. Genotoxicity were evaluated by counting and comparing the value of sperm abnormalities and normal sperm count. Results show that rats injected with CHB had remarkable increase in sperm head abnormalities as without hook, banana shape and hummer shape. Admission of cranberry extract and bone marrow cells after chemotherapy improved the frequency of the sperm abnormalities.

Key words: Chlorambucil, cranberry, bone marrow, sperm.