African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Antibacterial activity of water-phase extracts from bamboo shavings against food spoilage microorganisms

  Jianyou Zhang1,2 , Jinyan Gong1, Yuting Ding2 ,Baiyi Lu1, Xiaoqin Wu1 and Ying Zhang1*  
  1Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China. 2College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang Province, People’s Republic of China.  
Email: [email protected]

  •  Accepted: 05 October 2010
  •  Published: 08 November 2010

Abstract

 

Water-phase extract of bamboo shavings (WEBS), by supercritical carbon dioxide extraction, was evaluated for its antimicrobial action against the range of food borne and food spoilage pathogens using agar disc diffusion assay in nutrient agar and Czapek Dox Agar media. The WEBS exhibited antimicrobial activity againstStaphylococcus aureus, Bacillus subtilis, Escherichia coliAspergillus niger,Penicilliun citrinum and Saccharomyces cerevisiae with a concentration-dependent relationship. The minimum inhibitory concentrations (MICs) of the WEBS against the tested bacterial strains were found in the range of 4.9 - 32 mg/ml using the two-fold dilution method. Different heat treatment conditions have no significant influence on the antibacterial activity. Emodin was taken as the standard sample to test for the content of total anthraquinone compound and preliminarily verify its antibacterial mechanism, so as to lay a theoretical foundation for development of its natural preservatives.

 

Key words: Water-phase extract of bamboo shavings (WEBS), antimicrobial activity, natural preservative.

Abbreviation

Abbreviations: MICs, Minimum inhibitory concentrations; WEBS, water-phase extract of bamboo shavings; EBS, extract from bamboo shavings; EEBS, ethanol extract from bamboo shavings.