Proteomic techniques that allow the identification and quantification of stress-related proteins, mapping dynamics of their expression and post translational modifications represent an important approach in the research of plant stresses. Biotic stress is one of the major stresses limiting crop productivity and the geographical distribution of many important crops worldwide. Two hundred and seventeen protein spots reproducibly were detected from six gels by using two-dimensional electrophoresis. After tryptic digestion, MALDI-TOF/MS analysis and database searching of some of the identified proteins indicated that the proteins are known to be involved in several biotic stress related functions as disease associate with pathogens. Mass spectrometry analysis allowed the identification of 185 differential expressed proteins with isoforms including well known biotic stress responsive proteins. Keumgang (13%), Jinpum (8%), China-108 (14%), Yeonnon-67 (11%), Norin-61 (22%) and Kantou-107 (32%) were identified as biotic stress responses proteins directly coupled to disease and pathogen infection on wheat. Nevertheless, our studies provides new insights into identification of biotic stress responses protein in disease infected wheat grain by natural condition, the post-translational modification in protein sequences, verify eventual differences among the genotypes in relation to them, and demonstrates the advantages of proteomic analysis.
Key words: Biotic stress, matrix-assisted laser desorption ionization-time of flight, proteomics, post-translational modification, two-dimensional electrophoresis, wheat.
PR, Pathogenesis related; PTMs, posttrans-lational modifications;EDTA, ethylenediaminetetraacetic acid; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; CBB, Coomassie brilliant blue; ACN,acetonitrile; 2DE, dimensional gel electrophoresis; IEF, isoelectric focusing gels;MeJA, methyl jasmonate; JA, jasmonic acid; CSH, cystine-stabilized alpha-helical;SA, salicylic acid; CDPK, calcium-dependent protein kinase; ABA, abscisic acid;GA, gibberellic acid.
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