African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro antioxidant activity and inhibitory hepatic steatosis effect on oleic acid-induced fatty liver model of consecutive extracts from Rosa davurica Pall

  Ying Wei1, Muyi Cai1, Ruizeng Gu1, Jun Lu1, Feng Lin1 and Baoping Ji 2*      
  1China National Research Institute of Food and Fermentation Industries, Beijing 100027. 2College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People’s Republic of China, 100083.  
Email: [email protected]

  •  Accepted: 26 June 2013
  •  Published: 31 July 2013

Abstract

 

The nutrient and phytochemical composition of Rosa davurica Pall. fruit (RDF) from China were determined, including sugar, reducing sugar, ascorbic acid, caroteniods and phenolics. RDF was successively extracted with chloroform, ethyl acetate (EA), water-saturated n-butanol, ethanol and distilled water, respectively. Among the five fractions, EA fraction revealed the highest total phenolic content and the strongest antioxidant ability in cyclic voltammograms (CV) and oxygen radical absorbance capacity (ORAC) assays. Moreover, the strongest inhibitory activity against oleic acid-induced (OA-induced) fatty liver in vitro was also the EA fraction. Further, it was separated by a semi-preparative high performance liquid chromatography (HPLC) on C18 column. The most active fraction (EA-FII) of EA in all assays above, which mainly included catechin, quercetin-sedoheptulose and quercetin-94, was identified by LC/MS, HPLC and ultraviolet (UV)/V spectrum analysis.

 

Key words: RDF extracts, composition properties, antioxidant ability, inhibitory activity, oleic acid-induced, HPLC, LC/MS.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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