African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Chemical composition and antioxidant activity of Lippia alba essential oil obtained by supercritical CO2 and hydrodistillation

L. Reyes-Solano
  • L. Reyes-Solano
  • Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera A Eldorado Km 5.5. Col Campo el Diez. Culiacan, Sinaloa, Mexico.
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A. P. Breksa III
  • A. P. Breksa III
  • Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture. 800 Buchanan St. Albany, CA. USA.
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J. Benigno Valdez-Torres
  • J. Benigno Valdez-Torres
  • Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera A Eldorado Km 5.5. Col Campo el Diez. Culiacan, Sinaloa, Mexico.
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M. Angulo-Escalante
  • M. Angulo-Escalante
  • Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera A Eldorado Km 5.5. Col Campo el Diez. Culiacan, Sinaloa, Mexico.
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J. B. Heredia
  • J. B. Heredia
  • Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera A Eldorado Km 5.5. Col Campo el Diez. Culiacan, Sinaloa, Mexico.
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  •  Received: 17 February 2017
  •  Accepted: 12 April 2017
  •  Published: 26 April 2017

Abstract

Lippia alba essential oils were obtained by hydrodistillation (HD) and supercritical fluid (SFE) extraction methods. These were analyzed by gas chromatography-mass spectrometry-flame ionization detector (GC-FID-MS). Antioxidant activity was tested by DPPH and ABTS methods, and total soluble phenolics (TSP) were also determined. While in the SFE extract 14 compounds were identified (mostly: myrcenone 3.4%, α-terpineol 1.0% and β-caryophyllene 2.3%), in the HD extract 17 compounds were identified (mostly: eucalyptol 15.6%, myrcenone 9.3% and Z-ocimenone 5.8%). The results showed that L. alba essential oils obtained by SFE was IC50=17.35 mg/mL and by HD was IC50 = 12.45 mg/mL in DPPH assay. L. alba has an excellent eucalyptol content.

 

Key words: Antioxidants, bioactive compounds, supercritical fluid, green technologies.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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