African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12200

Full Length Research Paper

Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation

Boris Gutarra Castillo
  • Boris Gutarra Castillo
  • Laboratorio de Biotecnología, Universidad Continental. Av. San Carlos 1980, Código Postal 12000. Huancayo, Junín, Perú.
  • Google Scholar
Marina Díez-Municio
  • Marina Díez-Municio
  • Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Campus de Canto blanco. 28049, Madrid, España.
  • Google Scholar
Juan Cisneros de la Cruz
  • Juan Cisneros de la Cruz
  • Laboratorio de Biotecnología, Universidad Continental. Av. San Carlos 1980, Código Postal 12000. Huancayo, Junín, Perú.
  • Google Scholar
Javier Moreno
  • Javier Moreno
  • Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Campus de Canto blanco. 28049, Madrid, España.
  • Google Scholar


  •  Received: 26 May 2017
  •  Accepted: 04 August 2017
  •  Published: 30 August 2017

Abstract

A strain of Aspergillus niger PR-142 native to northern coast of Peru was subjected to successive processes of mutagenesis by ultraviolet light (UV) irradiation at 253.7 nm to increase the production of fructooligosaccharides (FOS). An initial selection was made by considering the mutants with increased invertase activity followed by the measurement of β-fructosyltransferase (FTase) activity both in mycelium and extracellular environment. Five selected mutants, which showed increased values of mycelium invertase activity (ranging from 101 to 128% as compared to the parent strain) at 40°C and sodium dodecylsulfate 0.15 (w/v), were  grown in a fermentative medium in 50 mL conical tubes on a rotary shaker, and their FTase activity was determined. The 6-M69 mutant showed the most active mycelium activity of 1.5 fold as compared to the parent strain. When the same reaction was performed between 1 to 4 h, at the 3rd h, the mycelium FTase activity significantly increased up to 7 and 3 times in the mutant and parental strain, respectively. Finally, 4 mutants and the parental PR-142 were genetically characterized using inter simple sequence repeat polymerase chain reaction (ISSR-PCR) molecular markers. This analysis showed a significant 33% polymorphic bands between the parent and mutant markers, and 20 bands were unique to the mutants.

Key words: Aspergillus, mutagenesis, β-fructosyltransferase, fructooligosaccharides, inter simple sequence repeat polymerase chain reaction (ISSR-PCR).