African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

Wenwen Zhao
  • Wenwen Zhao
  • Jiangsu Engineering and Technology Research Center of Microbiology Resource; Jiangsu Key Laboratory for Biodiversity and Biotechnology; College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Yuru Chen*
  • Yuru Chen*
  • Jiangsu Engineering and Technology Research Center of Microbiology Resource; Jiangsu Key Laboratory for Biodiversity and Biotechnology; College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Shaofeng Li
  • Shaofeng Li
  • Jiangsu Engineering and Technology Research Center of Microbiology Resource; Jiangsu Key Laboratory for Biodiversity and Biotechnology; College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Kewei Dai
  • Kewei Dai
  • School of Geography Science, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Yan Chen
  • Yan Chen
  • School of Geography Science, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Shijie Yang
  • Shijie Yang
  • School of Life Science and Environment, College of Yichun, Yichun, China.
  • Google Scholar


  •  Received: 08 March 2015
  •  Accepted: 15 May 2015
  •  Published: 20 May 2015

Abstract

Methods for separation of chlorogenic acid (CA) from tobacco by-products were established. The liquid chromatography tandem mass spectrometry (LC-MS) method for identification and analysis of chlorogenic acid from tobacco materials has been developed. CA was isolated by polyamide and further purified by silicagel column chromatography. Results reveal that polyamide is suitable for separation of CA from tobacco extract. After one run treatment with polyamide, the content of chlorogenic acids was 40.3%. The product was further purified using silicagel column chromatography; the content of total chlorogenic acid was increased 2.29-fold from 40.3 to 92.2%. The LC-MS results showed that total chlorogenic acids were made up of four components: 5-CQA, 3-CQA, 4-CQA and cis-5-CQA. The content of 5-CQA was the most (78.2%) and other three chlorogenic acid derivatives were 1.9, 10.1 and 2.0%, respectively.

 

Key words: Tobacco by-products, chlorogenic acid, polyamide, silica gel column, LC-MS.

Abbreviation

CA, Chlorogenic acid; LC-MS, liquid chromatography tandem mass spectrometry; electrospray ionization (ESI).