African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Separation and purification of chlorogenic acid from tobacco by-products by polyamide and silicagel column chromatography

Wenwen Zhao
  • Wenwen Zhao
  • Jiangsu Engineering and Technology Research Center of Microbiology Resource; Jiangsu Key Laboratory for Biodiversity and Biotechnology; College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Yuru Chen*
  • Yuru Chen*
  • Jiangsu Engineering and Technology Research Center of Microbiology Resource; Jiangsu Key Laboratory for Biodiversity and Biotechnology; College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Shaofeng Li
  • Shaofeng Li
  • Jiangsu Engineering and Technology Research Center of Microbiology Resource; Jiangsu Key Laboratory for Biodiversity and Biotechnology; College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Kewei Dai
  • Kewei Dai
  • School of Geography Science, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Yan Chen
  • Yan Chen
  • School of Geography Science, Nanjing Normal University, Nanjing, Jiangsu 210023, PR. China.
  • Google Scholar
Shijie Yang
  • Shijie Yang
  • School of Life Science and Environment, College of Yichun, Yichun, China.
  • Google Scholar


  •  Received: 08 March 2015
  •  Accepted: 15 May 2015
  •  Published: 20 May 2015

References

Chen JX, Leng HQ, Duan YX, Zhao W, Yang GY, Guo Y D, Chen YK, Hu QF (2013). Three new flavonoids from the leaves of oriental tobacco and their cytotoxicity. Phytochem. Lett. 6:144-147.
Crossref

 

Danino O, Gottlieb HE, Grossman S, Bergman M (2009). Antioxidant activity of 1,3-dicaffeoylquinic acid isolated from Inula viscosa. Food Res. Intl. 42:1273-1280.
Crossref

 
 

Guo YX, Zhou LL, Li T, Wang LH (2011). Preparative separation of lithospermic acid B from Saliva miltiorrhize by polyamide resin and preparative high-performance liquid chromatography. J. Chromatogr. A. 1218:4606-4611.
Crossref

 
 

He WH, Liu X, Xu HG, Gong Y, Yuan F, Gao YX (2010). On-line HPLC-ABTS screening and HPLC-DAD-MS/MS identification of free radical scavengers in Gardenia(Gardenia jasminoides Ellis) fruit extracts, Food Chem. 123:521-528.
Crossref

 
 

Li H, Chen B, Yao SZ (2005). Application of ultrasonic technique for extracting chlorogenic acid from Eucommia ulmodies Oliv.(E. ulmodies). Ultrasonic Sonochem. 12:295-300.
Crossref

 
 

Mazvimba MT, Yu Y, Cui ZQ, Zhang Y (2012). Optimization and orthogonal design of an ultrasonic-assisted aqueous extraction process for extracting chlorogenic acid from dry tobacco leaves, Chinese J. Nat. Med. 10:311-320.
Crossref

 
 

Rodrigues NP, Bragagnolo N (2013). Identification and quantification of bioactive compounds in coffee brews by HPLC-DAD-MSn, J. Food Composit. Analys. 32:105-115.
Crossref

 
 

Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, Sugawara M, Iseki K (2011). In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid, Intl. J. Pharm. 403:136-138.
Crossref

 
 

Sun LJ, Guo YR, Fu CC, Li JJ, Li Z (2013). Simultaneous separation and purification of total polyphenols, chlorogenic acid and phlorizin from thinned young apples, Food Chem. 136:1022-1029.
Crossref

 
 

Wan PF, Sheng ZL, Han Q, Zhao YL, Cheng GD, Li YH (2014). Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro. J. Chromatogr. B. 945-946:68-74.
Crossref

 
 

Wang GF, Shi LP, Ren YD, Liu QF, Liu HF, Zhang RJ, Li Z (2009). Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro. Antiviral Res. 83:186-190.
Crossref

 
 

Yuan XY, Gao MZ, Xiao HB, Tan CY, Du YG (2012). Free radical scavenging activities and bioactive substances of Jerusalem artichoke (Helianthus tuberosus L.) leaves. Food Chem. 133:10-14.
Crossref

 
 

Yun N, Kang JW, Lee SM (2012). Protective effects of chlorogenic acid against ischemia/reperfusion injury in rat liver: molecular evidence of its antioxidant and anti-inflammatory properities. J. Nutrition. Biochem. 23:1249-1255.
Crossref

 
 

Zhang B, Yang RY, Zhao Y, Liu CZ (2008). Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins. J. Chromat. B. 867:253-258.
Crossref

 
 

Zhang JY, Zhang Q, Li N, Wang ZJ, Lu JQ, Qiao YJ (2013). Diagnostic fragment-ion-based and extension strategy coupled to DFls intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPC-ESI-MSn, Talanta. 104:1-9.
Crossref

 
 

Zhang XZ, Gao HJ, Zhang LF, Liu DH, Ye XQ (2012). Extraction of essential oil from discarded tobacco leaves by solvent extraction and steam distillation, and identification of its chemical composition. Industrial Crops Prod. 39:162-169.
Crossref

 
 

Zhao M, Wang HY, Yang B, Tao H (2010). Identification of cyclodextrin inclusion complex of chlorogenic acid and its antimicrobial activity. Food Chem. 120:1138-1142.
Crossref