African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Biosynthesis of silver nanoparticles by plants crude extracts and their characterization using UV, XRD, TEM and EDX

Vishwajeet Singh*
  • Vishwajeet Singh*
  • Department of Botany, Raja Balwant Singh, College, Dr B.R. Ambedkar University, Agra-282002, U.P., India.
  • Google Scholar
Ankita Shrivastava
  • Ankita Shrivastava
  • New Era Research Foundation, Agra-282007, India, U.P., India.
  • Google Scholar
Nitin Wahi
  • Nitin Wahi
  • Department of Biotechnology, GLA University, Mathura-281406, U.P., India.
  • Google Scholar

  •  Received: 04 May 2015
  •  Accepted: 13 July 2015
  •  Published: 19 August 2015


Plant extracts are very cost effective and eco-friendly, thus, can be an economic and efficient alternative for the large-scale synthesis of nanoparticles. The preparation of stable, uniform silver nanoparticles by reduction of silver ions with Emblica officinalis, Terminalia catappa and Eucalyptus hybrida extract is reported in the present paper. It is a simple process of global research interest for obtaining silver nanoparticles in least amount of time. These nanoparticles were characterized with UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy diffraction X- ray (EDX analysis which revealed that the silver nanoparticles are polydisperse and of different morphologies ranging from 20 to 80 nm in size. XRD results reveal that these nanostructures exhibit a face-centered cubic crystal structure. The UV/Vis spectra absorption peak confirms their production. Pioneering of reliable and eco-friendly process for synthesis of metallic nanoparticles biologically is an important step in the field of application of nanobiotechnology. Thus, these silver nanoparticles (Ag-NPs) may prove as a better candidate for drugs and can potentially eliminate the problem of chemical agents because of their biogenic nature. The indiscriminate use of antibiotics has fuelled the development of drug resistance at an alarming rate. To overpower this burning problem, the Ag-NPs may prove to be a universal solution.


Key words: Nanobiotechnology, silver nanoparticles (Ag-NPs), Emblica officinalis, Terminalia catappa and Eucalyptus hybrida.