African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Full Length Research Paper

Micropropagation of Peach, Prunus persica (L.) BATSCH. cv. Garnem

Wegayehu Felek*
  • Wegayehu Felek*
  • Department of Plant Science, College of Agriculture and Natural Resource Science, Debre Berhan University, Ethiopia.
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Firew Mekibib
  • Firew Mekibib
  • Department of Plant Science, College of Agriculture and Environmental Science, Haramaya University, Ethiopia.
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Belayneh Admassu
  • Belayneh Admassu
  • Plant Tissue Culture Laboratory, National Agricultural Biotechnology Laboratory, Holeta Agricultural Research Center, Ethiopia.
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  •  Received: 06 January 2016
  •  Accepted: 09 June 2016
  •  Published: 08 March 2017

Abstract

The present study aimed to develop micropropagation procedure for Prunus persica cv. Garnem. Shoot induction and multiplication was found from nodal explants cultured on MS medium fortified with different concentrations of two cytokinins (6-benzyladeninepourine and Kinetin) alone and in combination with 0.5 mg/l gibberellic acid and 0.01 mg/l indole-3-butyric acid. Microplantlets were cultured to root on ½ strength MS medium supplemented with different concentrations of indole-3-butyric acid and α-naphthalene-acetic acid alone. 6-Benzyladeninepourine (0.5 mg/l) with indole-3-butyric acid (0.01 mg/l) and gibberellic acid (0.5 mg/l) was found to be the optimum combination treatment for shoot initiation (100%). 6-Benzyladeninepourine (2.0 mg/l) with indole-3-butyric acid (0.01 mg/l) and gibberellic acid (0.5 mg/l) was found to be optimum for maximum shoot number per explant (7.67). The maximum rooting (42.86%), maximum root number/shoot (6.33) and longest rooting (7.17 cm) were found at 1.5 mg/l indole-3-butyric acid. α-naphthalene-acetic acid was found to be not effective. The plantlets were acclimatized in the glasshouse and survival percentage was 73.3% at potting mixture of autoclaved river sand, forest soil and manure in a 2:1:1 (v/v/v) ratio.

Key words: Acclimatization, auxin, cytokinin, micropropagation, Prunus persica.