African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Physicochemical properties of rhodanese from the kidney of cane rat (Thryonomys swinderianus)

Leonard Ona Ehigie
  • Leonard Ona Ehigie
  • Department of Biochemistry, Faculty of Basic Medical Science, Ladoke Akintola University Technology, Ogbomoso, Nigeria.
  • Search for this author on:
  • Google Scholar
Bamidele Sanya Fagbohunka
  • Bamidele Sanya Fagbohunka
  • Department of Biochemistry, Faculty of Basic Medical Science, Olabisi Onabanjo University, Remo Campus, Ikenne, Ogun State, Nigeria.
  • Search for this author on:
  • Google Scholar
Babamotemi Oluwasola Itakorode
  • Babamotemi Oluwasola Itakorode
  • Department of Chemical Sciences, College of Natural and Applied Sciences, Oduduwa University Ipetumodu, Ile-Ife, Nigeria.
  • Search for this author on:
  • Google Scholar
Raphael Emuebie Okonji
  • Raphael Emuebie Okonji
  • Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
  • Search for this author on:
  • Google Scholar


  •  Received: 13 April 2019
  •  Accepted: 31 May 2019
  •  Published: 12 June 2019

Abstract

We report the properties of partially purified rhodanese, a cyanide detoxifying enzyme isolated from the kidney of the greater cane rat commonly known as grasscutter (Thryonomys swinderianus). T. swinderianus kidney rhodanese had a specific activity of 1.43 µmole/min/mg protein, a fold of 2.31 and a 55% recovery. The apparent Km values of 0.408 and 0.316 mM were obtained for both sodium thiosulphate (Na2S2O3) and potassium cyanide (KCN) respectively. Substrate specificity study showed that T. swinderianus rhodanese can use other substrates. The enzyme showed its maximum activity at pH 8.0 and 50°C. The assay for the effect of metal ions showed that the enzyme is not affected or inhibited by metal ions such as Na+, Fe3+ but inhibited by Hg2+. The continued existence of T. swinderianus after consumption of food crops with high cyanogenic glycosides suggests that the animal has a functional cyanide detoxification mechanism.

 

Keywords: Rhodanese, cyanide, kinetics, detoxification, cane rat, grasscutter.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 18 No. 24
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES