African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Full Length Research Paper

Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties

Ethel N. Webi
  • Ethel N. Webi
  • International Potato Center, Biosciences East and Central Africa (BecA-ILRI hub), P. O. Box 25171-00603 Nairobi, Kenya.
  • Google Scholar
Kariuki Daniel
  • Kariuki Daniel
  • Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200 Nairobi, Kenya.
  • Google Scholar
Kinyua Johnson
  • Kinyua Johnson
  • Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200 Nairobi, Kenya.
  • Google Scholar
Njoroge Anne
  • Njoroge Anne
  • International Potato Center, Biosciences East and Central Africa (BecA-ILRI hub), P. O. Box 25171-00603 Nairobi, Kenya.
  • Google Scholar
Ghislain Marc
  • Ghislain Marc
  • International Potato Center, Biosciences East and Central Africa (BecA-ILRI hub), P. O. Box 25171-00603 Nairobi, Kenya.
  • Google Scholar
Magembe Eric
  • Magembe Eric
  • International Potato Center, Biosciences East and Central Africa (BecA-ILRI hub), P. O. Box 25171-00603 Nairobi, Kenya.
  • Google Scholar


  •  Received: 13 May 2019
  •  Accepted: 29 August 2019
  •  Published: 30 September 2019

References

Ahmad ZM, Hussain I, Muhammad A, Ali S, Ali MG, Roomi S, Zia AM, Ijaz A (2012). Factor affecting Agrobacterium-mediated transformation of rice chitinase gene in Solanum tuberosum L. African Journal of Biotechnology 11(41):9716-9723.
Crossref

 

Alice MQ, Joy B (2016). Modulation of R-gene expression across environments. Journal of Experimental Botany 67(7): 2093-2105
Crossref

 
 

Chambers JA, Zambrano P, Falck-Zepeda JB, Gruère GP, Sengupta D, Hokanson K (2014). GM agricultural technologies for Africa: a state of affairs. International Food Policy Research Institute.

 
 

Cooke D, Lees A, Shaw D, Ruairidh B, Cooke L (2006). Variation in aggressiveness in Phytophthora infestans . British potato council. 

 
 

Cuellar W, Gaudin A, Solórzano D, Casas A, Ñopo L, Chudalayandi P, Medrano G, Kreuze J, Ghislain M (2006). Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato. Plant molecular biology 62(1-2):71-82.
Crossref

 
 

Devaux A, Kromann P, Ortiz O (2014). Potatoes for sustainable global food security. Potato Research 57(3-4):185-199.
Crossref

 
 

EPA Ireland (2006). Response from BASF to request further information, publicly.

 
 

Gawel NJ, Jarret RI (1991). A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Molecular Biology Reporter 9(3):262-266.
Crossref

 
 

Ghislain M, Arinaitwe AB, Magembe E, Njoroge A, Rivera C, Román ML, Tovar JC, Gamboa S, Forbes GA, Kreuze JF, Barekye A, Kiggundu A (2018). Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races. Plant Biotechnology Journal 17(6):1119-1129.
Crossref

 
 

Haesaert G, Vossen HJ, Custers R, Loose M, Haverkort A, Heremans B, Hutten R, Kessel G, Landschoot S, Droogenbroeck B, Visser R, Gheysen G (2015). Transformation of the potato variety Desiree with single or multiple resistance genes increases resistance to late blight under field conditions. Crop protection 77:163-175.
Crossref

 
 

Hannukkala AO, Kaukoranta T, Lehtinen A, Rahkonen A (2007). Late blight epidemics on potato in Finland 1933-2002; increased and earlier occurrence of epidemics associated with climate change and lack of rotation. Plant Pathology 56(1):167-176.
Crossref

 
 

Haverkort A, Struik P (2015). Yield levels of potato crops: recent achievements and future prospects. Field Crops Research 182:76-85.
Crossref

 
 

Haverkort AJ, Struik PC, Visser RGF, Jacobsen E (2009). Applied Biotechnology to Combat Late Blight in Potato Caused by Phytophthora Infestans. Potato Research 52(3):249-264.
Crossref

 
 

Heeres P, Schippers-Rozenboom M, Jacobsen E, Visser RG (2002). Transformation of a large number of potato varieties: genotype-dependent variation in efficiency and somaclonal variability. Euphytica 124(1):13-22.
Crossref

 
 

Hood EE, Gelvin SB, Melchers LS, Hoekema A (1993). New Agrobacterium helper plasmids for gene transfer to plants (EHA105). Transgenic Research 2(4):208-218.
Crossref

 
 

International Monetary Fund (IMF) (2012). International Jobs Report, Economist Intelligence Unit, Washington, DC.

 
 

International Year of the Potato (IYP) (2008). The Potato United Nations Food and Agricultural Organization Retrieved 6 August 2015.

 
 

Kaguongo W, Nyangweso A, Mutunga J, Nderitu J (2013). A policymakers' Guide to Crop Diversification: The Case of the Potato in Kenya, FAO, Rome. 

View

 
 

Knapova G, Gisi U (2002). Phenotypic and genotypic structure of Phytophthora infestans populations on potato and tomato in France and Switzerland. Plant Pathology 51(5):641-653.
Crossref

 
 

Kramer LC, Choudoir MJ, Wielgus SM, Bhaskar PB, Jiang J (2009). Correlation between transcript abundance of the RB gene and the level of the RB-mediated late blight resistance in potato.  Molecular Plant-Microbe Interactions 22(4):447-455.
Crossref

 
 

Laititi M (2014). Potato market survey in Kenya: An agricultural product value chain approach. International Journal of Business and Management Review 2(6):59-87.

 
 

Millet BP, Mollov DS, Lorrizo M, Carputo D, Bradeen JM (2009). Changes in disease resistance phenotypes associated with plant physiological age are not caused by variation in R gene transcript abundance. Molecular Plant-Microbe Interactions 22(3):362-368.
Crossref

 
 

Millet BP, Gao L, Lorrizo M, Carputo D, Bradeen JM (2015). Potato tuber blight resistance phenotypes correlate with RB transgene transcript levels in an age-dependent manner. Phytopathology 105(8):1131-1136.
Crossref

 
 

Muthoni J, Shimelis H, Melis R (2013). Potato production in Kenya: Farming systems and production constraints. Journal of Agricultural Science 5(5):182-197.
Crossref

 
 

National Potato Council of Kenya (NPCK) (2015). Potato Variety Catalogue Retrieved from the National Potato Council of Kenya Website. 

View

 
 

New Partnership for Africa's Development (NEPAD) (2013). Agriculture in Africa: Transformation and Outlook: Prepared by Roger Blein, Martin Bwalya, Sloans Chimatiro, Benoît Faivre-Dupaigre, Simon Kisira, Henri Leturque and Augustin Wambo-Yamdjeu 15 p.

 
 

Njoroge A, Tusiime G, Forbes G, Yuen J (2016). Displacement of US‐1 clonal lineage by a new lineage of Phytophthora infestans on potato in Kenya and Uganda. Plant Pathology 65(4):587-592.
Crossref

 
 

Oh S-K, Kim H, Choi D (2014). Rpi-blb2-mediated late blight resistance in Nicotiana benthamiana requires SGT1 and salicylic acid-mediated signaling but not RAR1 or HSP90. FEBS Letters 588(7):1109-1115.
Crossref

 
 

Oh S-K, Young C, Lee M, Oliva R, Bozkurt TO, Cano LM, Win J, Bos JI, Liu HY, van Damme M, Morgan W, Choi D, van der Vossen EA, Vleeshouwers VG, Kamoun S (2009). In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes, including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2. The Plant Cell 21:2928-2947.
Crossref

 
 

Orbegozo J, Roman ML, Rivera C, Gamboa S, Tovar JC, Forbes GA, Lindqvist-Kreuze H, Kreuze JF, Ghislain M (2016). Rpi-blb2 gene from Solanum bulbocastanum confers extreme resistance to late blight disease in potato. Plant Cell, Tissue and Organ Culture 125(2):69-281.
Crossref

 
 

Roman ML, Izarra M, Lindqvist-Kreuze H, Rivera C, Gamboa S, Tovar JC, Kreuze JF, Forbes GA, Ghislain M (2017). R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1. Plant Cell, Tissue and Organ Culture 131(2):259-268.
Crossref

 
 

Schumann GL, D'Arcy CJ (2005). Late blight of potato and tomato. The Plant Health Instructor 724-733.
Crossref

 
 

Sharma BP, Forbes AG, Manandhar KH, Shrestha SM, Thapa RB (2013). Determination of Resistance to Phytophthora infestans on Potato Plants in Field, Laboratory and Greenhouse Conditions. Journal of Agricultural Science 5(5):9752-9760 ISSN 1916-9752E-ISSN 1916-9760.
Crossref

 
 

Ssengooba T, Hakiza JJ (1999). The current status of late blight caused by Phytophthora infestans in Africa, with emphasis on eastern and southern Africa. Proceedings of Global Initiative on Late Blight Conference, March 16-19 Quito, Ecuador pp. 25-28.

 
 

Vleeshouwers VGA, Dooijeweert W, Govers F, Kamoun S, Colon TL (2000). The hypersensitive response is associated with host and non-host resistance to Phytophthora infestans. Planta 210(6):853-864.
Crossref

 
 

Vleeshouwers VG, Raffaele S, Vossen JH, Champouret N, Oliva R, Segretin ME, Pel MA (2011). Understanding and exploiting late blight resistance in the age of effectors. Annual Review of Phytopathology 49:507-531.
Crossref

 
 

Wurster R (1977). Social science research and training: report of the planning conference on social science research and training held at CIP-Lima, Peru, August 8-12, 1977. Lima: Centro Internacional de la papa.

 
 

Yuen JE, Forbes GA (2009). Estimating the level of susceptibility to Phytophthora infestans in potato genotypes. Phytopatholog 99(6):782-786.
Crossref

 
 

Zhu S, Li Y, Vossen JH, Visser GRF, Jacobsen E (2012). Functional stacking of three resistance genes against Phytophthora infestans in potato. Transgenic Research 21(1):89-99.
Crossref