African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

Mercy Sinkiyian Kepue
  • Mercy Sinkiyian Kepue
  • Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Cecilia M. Mweu
  • Cecilia M. Mweu
  • Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
David Muruu Kariuki
  • David Muruu Kariuki
  • Biosciences Eastern and Central Africa, International Livestock Research Institute, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar
Linnet Kerubo
  • Linnet Kerubo
  • International Institute of Tropical Agriculture, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar
Isaac Njaci
  • Isaac Njaci
  • Biosciences Eastern and Central Africa, International Livestock Research Institute, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar
Anne Njoroge
  • Anne Njoroge
  • Biosciences Eastern and Central Africa, International Livestock Research Institute, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar
Leah Kago
  • Leah Kago
  • Biosciences Eastern and Central Africa, International Livestock Research Institute, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar
Benard O. Mware
  • Benard O. Mware
  • International Institute of Tropical Agriculture, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar
Sita R. Ghimire
  • Sita R. Ghimire
  • Biosciences Eastern and Central Africa, International Livestock Research Institute, P. O. Box 30709-00100, Nairobi, Kenya.
  • Google Scholar


  •  Received: 13 March 2020
  •  Accepted: 24 June 2021
  •  Published: 31 July 2021

References

Addinsoft (2021). XLSTAT statistical and data analysis solution. New York, USA.

View

 

Asghar S, Ahmad T, Hafiz IA, Yaseen M (2011). In vitro propagation of orchid (Dendrobium nobile) var.Emma white. African Journal of Biotechnology 10(16):3097-3103.
Crossref

 
 

Behera KK, Sahoo S (2008). In vitro micropropagation of Colocasia esculenta (L.) Schott. (cv local-jhankhri) through corm sprouts. The Orissa Journal of Horticulture 36:50-54.

 
 

Boampong R, Aboagye LM, Nyadanu D, Essilfie ME, Adu Amoah R (2019). Biochemical characterization of some Taro (Colocasia esculenta L. Schott) Germplasm in Ghana. Advances in Agriculture. 
Crossref

 
 

Burner DM, Grisham MP (1995). Induction and stability of phenotypic variation in sugarcane as affected by propagation procedure. Crop Science 35(3):875-880.
Crossref

 
 

Bhojwani SS, Dantu PK (2013). Micropropagation. In: Plant Tissue Culture: An Introductory Text. Springer, India.
Crossref

 
 

Chand H, Pearson MN, Lovell PH (1999). Rapid vegetative multiplication in Colocasia esculenta (L.) Schott (Taro). Plant Cell, Tissue and Organ Culture 55(3):223-226.
Crossref

 
 

Chung RC, Goh CJ (1994). High frequency direct shoot regeneration from corm axillary buds and rapid clonal propagation of Taro, Colocasia esculenta var. esculenta (L.) Schott (Araceae). Plant Science 104(1):93-100.
Crossref

 
 

El-sayed SE, Gharib AA, El-Sawy AM, Omaima SD (2016). Micropropagation protocol of Egyptian native cultivar of Taro, Colocasia esculenta var. esculenta. International Journal of Advanced Research in Biological Sciences 3(1):17-26.

 
 

Fay MF (1992). Conservation of rare and endangered plants using In vitro methods. In Vitro Cellular and Developmental Biology-Plant 28(1):1-4.
Crossref

 
 

Food and Agriculture Organization of the United Nations (2021). FAOSTAT statistical database. Rome: FAO.

 
 

Gupta N, Jain V, Rosy M, Dev S (2020). A Review on Micropropagation Culture Method, Asian Journal of Pharmaceutical Research and Development 8(1):86-93.
Crossref

 
 

Hossain MJ (2012). In vitro organogenesis of Colocasia esculenta cv. Antiquorum L. American Journal of Plant Sciences 3:709-713.
Crossref

 
 

International Atomic Energy Agency (IAEA) (2004). Low cost options fortissue culturetechnology in developing countries. Proceedings of a Technical Meeting organized by theJoint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, 26-30 August 2002, Vienna. IAEA-TECDOC-1384,.102p.

 
 

Jackson GV, Vilsoni F, Kumar J, Pearson MN, Morton JR (2001). Comparison of the growth of In vitro produced pathogen-tested Colocasia taro and field-collected planting material. New Zealand Journal of Crop and Horticultural Science 29:171-176.
Crossref

 
 

Jane J, Shen L, Lim S, Kasemsuwantt T, Nip K (1992). Physical and chemical studies of taro starches and flours. Cereal Chemistry 69(5):528-535.

 
 

Kantaka S (2004). Colocasia esculenta (L.). Schott. Grubbrn GJH, Denton OA (Eds). PROTA (Plant resources of Tropical Africa / Ressources vegetales de l' Afrique tropicale). Netherlands, Wageningen.

 
 

Kidanemariam DB (2018). Viruses of taro and other edible aroids in east Africa (Doctoral dissertation, Queensland University of Technology).

 
 

Lebot V (2009). Tropical root and tuber crops: cassava, sweet potato, yams and aroids. UK, MPG Biddles Ltd.

 
 

Liu C, Zhu J, Liu Z, Li L, Pan R, Jin L (2002). Exogenous auxin effects on growth and phenotype of normal and hairy roots of Pueraria lobata (Wild.) Ohwi. Plant Growth Regulation 38:37-43.
Crossref

 
 

Manju B, Evelyn, Mbong AG, Fokunang N, Charles Tembe-Fokunang, Estella A, Rachid H (2017). Application of In vitro micropropagation technique for sustainable production of four local taro cultivars in Cameroon. African Journal of Biotechnology 16(30):1638-1645.
Crossref

 
 

Matthews PJ (1991). A possible tropical wild type taro: Colocasia esculenta var. equatilis. Indo-Pacific Prehistory Association Bulletin 11:69-81.
Crossref

 
 

Minas GJ (2002). In vitro micropropagation of kolokasi from apical meristem. Agricultural Research Institute, Nicosia, Cyprus. Miscellaneous Reports 84. 8 p.

 
 

Mukami A, Ngetich A, Mweu C, Muthangya M, Oduor RO, Ngugi M, Mbinda W (2018). "Rapid and Efficient Plant Regeneration from Shoot Apical Meristems of Finger Millet [ Eleusine Coracana (L .) Gaertn]. African Journal of Biotechnology 17(29):898-905.
Crossref

 
 

Ngetich A, Runo S, Omwoyo O, Ngugi M (2015). "Low-Cost Micropropagation of Local Varieties of Taro (Colocasia esculenta Spp .)". Biotechnology Journal International pp. 136-145.
Crossref

 
 

Okonkwo CAC (1993). Taro: Colocasia spp. In: G Kalloo, BO Bergh (eds.) Genetic Improvement of Vegetable Crops pp. 709-715. Pergamon, Amsterdam.
Crossref

 
 

Onwueme IC (1999). Taro cultivation in Asia and the Pacific. Bangkok, Thailand, Food and Agriculture Organization of the United Nations Regional Office for Asia and the Pacific.

 
 

Ozel CA, Khawar KH, Mirici S, Arslan O, Sebahattin O (2006). Induction of Ex Vitro Adventitious Roots on Soft Wood Cuttings of Centaurea tchihatcheffii tchihatcheffii Fisch et. Mey using Indole 3Butyric Acid and α-Naphthalene Acetic Acid. International Journal of Agriculture and Biology 8(1):66-69.

 
 

Ramakrishnan M, Ceasar SA, Duraipandiyan V, Ignacimuthu S (2014). Efficient plant regeneration from shoot apex explants of maize (Zea mays) and analysis of genetic fidelity of regenerated plants by ISSR markers. Plant Cell, Tissue, and Organ Culture 119(1):183-196.
Crossref

 
 

Sarasan V, Cripps R, Ramsay MM, Atherton C, McMichen M, Prendergast G, Rowntree JK (2006). Conservation in vitro of threatened plants -Progress in the past decade. In Vitro Cellular and Developmental Biology-Plant 42(3):206-214.
Crossref

 
 

Schiefelbein JW, Masucci JD, Wang H (1997). Building a root: The control of patterning and morphogenesis during root development. Plant Cell 9(7):1089-1098.
Crossref

 
 

Seetohul S, Puchooa D, Ranghoo-Sanmukhiya VM (2008). Genetic improvement of Taro (Colocasia esculenta var esculenta) through In vitro mutagenesis. University of Mauritius Research Journal 13:79-89.

 
 

Singh D, Jackson G, Hunter D, Fullerton R,Lebot V, Taylor M, Iosefa T, Okpul T, Tyson J (2012). "Taro Leaf Blight-A Threat to Food Security. Agriculture 2(4):182-203.
Crossref

 
 

Temesgen M, Retta N (2015). Nutritional potential, health and food security benefits of taro Colocasia esculenta (L.): A review. Food Science and Quality Management 36:23-30.

 
 

Toledo J, Espinoza N, Golmirzaie A (1998). Tissue culture management of in-vitro plantlets in potato seed production. Training Manual. International Potato Centre.

 
 

Tuia VS (1997). In vitro multiplication of taro (Colocasia esculenta var.esculenta L. Schott), M.Agric. Thesis, University of the South Pacific, Fiji.

 
 

Villalobos VM, Engelmann F (1995). Ex situ conservation of plant germplasm using biotechnology. World Journal of Microbiology and Biotechnology 11:375-382.
Crossref

 
 

Wang QC, Valkonen JP (2008). Efficient elimination of sweetpotato little leaf phytoplasma from sweetpotato by cryotherapy of shoot tips. Plant Pathology 57(2):338-347.
Crossref

 
 

Wang QC, Valkonen JP (2009). Cryotherapy of shoot tips: Novel pathogen eradication method. Trends in Plant Science 14(3):199-122.
Crossref

 
 

Wickham H (2016). ggplot2: Elegant Graphics for Data Analysis: Springer-Verlag New York.
Crossref

 
 

Yam TW, Hsu GI, Arditti J (1990). Plant regeneration in vitro of South Pacific taro (Colocasia esculenta var. esculenta cv. Akalomamale, Aracea) Plant Cell Reports 9:229-232.
Crossref

 
 

Yokoya NS, Hirotaka K, Hideki O, Takao K (1999). Effects of environmental factors and plant growth regulators on growth of the red alga Gracilaria vermiculophylla from Shikoku Island, Japan. Hydrobiologia 398:339-347.
Crossref

 
 

Yoshino H (2002). Morphological and genetic variation in cultivated and wild taro. In S Yoshida, PJ Matthews (Eds.), Vegeculture in east-ern Asia and Oceania (pp. 95-116). JCAS Symposium Series 16. Japan Center for Area Studies.