African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 384

Full Length Research Paper

The interplay between paraoxonase-1 and epigenetic changes in colorectal carcinoma

El-Tokhy Amira Kamel
  • El-Tokhy Amira Kamel
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Zin El-deen Doaa Hussein
  • Zin El-deen Doaa Hussein
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Foda Abd allah Mahmoud
  • Foda Abd allah Mahmoud
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Moussa Gamal Ibrahim
  • Moussa Gamal Ibrahim
  • Department of Gastrointestinal and Laparoscopic Surgery, Faculty of Medicine, Tanta University, Egypt.
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Abo El-Noeman Saad El-Din Abd el-fatah
  • Abo El-Noeman Saad El-Din Abd el-fatah
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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  •  Received: 09 April 2018
  •  Accepted: 16 August 2018
  •  Published: 31 August 2018

Abstract

Colorectal cancer (CRC) is the third leading cause of cancer-related death. Colorectal mucosa has high levels of oxidative stress (OS) landmarks. OS has deleterious effect on cell structures as lipids, proteins and DNA. Markers of protein oxidation are named as advanced oxidation protein products (AOPPs) which have been considered as novel disease biomarkers. OS may also induce DNA damage which leads to mutation of chromosomes. Paraoxonase 1 (PON1) and Arylesterase (ARE) metabolize different substrates and act as antioxidant enzymes. Disruption of epigenetic processes can lead to altered gene function and malignant transformation. Altered expression of genes that encode histone deacetylase (HDACs) are linked to tumor development. The aim of this study was to evaluate the PON1 and ARE activities in CRC patients and to assess the epigenetic fingerprint via estimation of HDAC. The study was carried out on 30 CRC patients. After excision of tumor, segments were divided into two groups: group A (Control group) included parts taken from safety margin; Group B: (CRC group) subdivided into 3 grades, grade I: well differentiated tumor, grade II: moderately differentiated tumor, and grade III: poor differentiated tumor. CRC groups showed elevation of OS landmarks (AOPPs and DNA damage), HDACs activity and significant decrease in the level of PON1 and activities of PON1 and ARE. The histopathological results were correlated with the laboratory results. It was concluded that the damage of AOPPs and DNA is novel biomarkers in diagnosis of CRC; PON1 is important protective antioxidant enzyme and HDAC activity estimation is a promising line in CRC diagnosis.

 

Key words: Colorectal cancer, epigenetics, oxidative stress, advanced oxidation protein products, paraoxonase 1, arylesterase, histone deacetylase enzyme.