African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 384

Full Length Research Paper

Prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency (A-376/202) in homozygous sickle cell patients in Senegal and its clinical impact

Dominique Doupa
  • Dominique Doupa
  • Service de Biochimie, Université Gaston Berger de Saint-Louis (UGB), Sénégal.
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Ousseynou Boye
  • Ousseynou Boye
  • Service de Biochimie, Université Gaston Berger de Saint-Louis (UGB), Sénégal.
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Maguette Ndiaye
  • Maguette Ndiaye
  • Service de Parasitologie, Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Mamadou Djité
  • Mamadou Djité
  • Service de Biochimie, Pharmaceutique Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Pape Matar Kandji
  • Pape Matar Kandji
  • Service de Biochimie, Pharmaceutique Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Sidy Mohamed Seck
  • Sidy Mohamed Seck
  • Service de Biochimie, Université Gaston Berger de Saint-Louis (UGB), Sénégal.
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Demba Makalou
  • Demba Makalou
  • Service de Biochimie, Université Gaston Berger de Saint-Louis (UGB), Sénégal.
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Aminata Lam
  • Aminata Lam
  • Service de Parasitologie, Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Marie Pierre Diouf
  • Marie Pierre Diouf
  • Service de Parasitologie, Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Arame Ndiaye
  • Arame Ndiaye
  • Service de Biochimie, Médicale Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Blaise Felix Faye
  • Blaise Felix Faye
  • Service de Parasitologie, Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Souleymane Thiam
  • Souleymane Thiam
  • Service de Biochimie, Médicale Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Abdourahmane Samba
  • Abdourahmane Samba
  • Service de Biochimie, Médicale Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Fatou Diallo
  • Fatou Diallo
  • Service de Biochimie, Médicale Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Papa Madieye Gueye
  • Papa Madieye Gueye
  • Service de Biochimie, Pharmaceutique Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Alassane Diatta
  • Alassane Diatta
  • Service de Biochimie, Médicale Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Babacar Faye
  • Babacar Faye
  • Service de Parasitologie, Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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Ibrahima Diagne
  • Ibrahima Diagne
  • Service de Biochimie, Université Gaston Berger de Saint-Louis (UGB), Sénégal.
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Saliou Diop
  • Saliou Diop
  • Service d’Hématologie, Université Cheikh Anta Diop Dakar (UCAD), Sénégal.
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  •  Received: 14 March 2018
  •  Accepted: 11 May 2018
  •  Published: 31 July 2018

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common worldwide enzymopathy with approximately 400 million individuals affected. This inherited disease is sex-linked recessive inheritance. The high prevalence of certain variants of G6PD in different populations and ethnic groups increases the likelihood of finding associations with other pathologies. Sickle cell disease and thalassemia are the most common pathologies associated with G6PD deficiency. The aim of this study was firstly to study the prevalence of glucose-6-phosphate dehydrogenase deficiency (A-376/202) by molecular analysis in homozygous sickle cell patients, and secondly to study the influence of this association on the clinical severity of the disease. In a cross-sectional study, 100 patients aged 15 years with homozygous sickle cell disease in the stationary phase regularly monitored in a National Center for Blood Transfusion were included over a six-month period stretching from September 2015 to February 2016. An EDTA sampling tube was taken from each patient for the study of hematological parameters and a molecular study for the detection of mutations 376 and 202. Clinical, epidemiological and biological variables were collected using a questionnaire. Data was analyzed using Epi-info 7.2. The results of the study showed that the variant A- characterized by a double mutation (376/202) was found with a frequency of 13% (13/100) with a clear male predominance (p ˂ 0.006). Variant A- was statistically significantly associated with cholelithiasis (p˂0.031). This study is of therapeutic interest since the recognition of G6PD-deficient sickle cell disease would make it possible to take adequate preventive measures with respect to the taking of oxidizing drugs.

Key words: Glucose-6-phosphate dehydrogenase (G6PD) deficiency, sickle cell disease, stationary phase, Sénégal.