African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 381

Full Length Research Paper

Evaluation of the effect of vitamin C on caspase 9 and oxidative stress in rheumatoid arthritis patients

Amira M. Elshamy
  • Amira M. Elshamy
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Nagah K. Gaafar
  • Nagah K. Gaafar
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Nadia E. ElAshwah
  • Nadia E. ElAshwah
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Ayman A. Wagih
  • Ayman A. Wagih
  • Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Egypt.
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Abeer A. Shahba
  • Abeer A. Shahba
  • Department of Internal Medicine, Faculty of Medicine, Tanta University, Egypt.
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  •  Received: 08 May 2018
  •  Accepted: 03 September 2018
  •  Published: 30 November 2018

Abstract

Rheumatoid arthritis (RA) is a chronic and an autoimmune disease of the joints and is widely distributed worldwide. It is characterized by alterations of the antioxidant defense system and increased free radical formation and pro-inflammatory cytokine. The aim of the present study was to evaluate the effect of vitamin C supplementation on oxidative stress biomarkers and caspase 9 level in rheumatoid arthritis patients. This study included 30 RA patients and 30 healthy subjects. Plasma levels of malondialdehyde (MDA), total antioxidant capacity (TAC), caspase 9 and 8-hydroxy-2′-deoxyguanosine (8 OHdG) were assayed as well as blood vitamin C level. These parameters were re-evaluated in RA patients after vitamin C supplementation for one month. Increased MDA and 8 OHdG levels and reduced TAC, caspase 9 and vitamin C. Levels were demonstrated in RA patients. After vitamin C supplementation, RA patients showed significant increase in TAC and vitamin C level and significant decrease in MDA and 8 OHdG levels, plasma caspase 9 level was not significantly affected after vitamin C supplementation. Increased oxidative stress and decreased apoptosis may have an important role in the pathogenesis of RA. The administration of vitamin C supplementation may help to relieve oxidative stress and enhance the antioxidant defense in these patients.

 

Key words: Rheumatoid arthritis, oxidative stress, apoptosis, caspase 9, vitamin C.