Studies on some physicochemical properties of Rhodanese synthesized by Bacillus cereus isolated from the effluents of iron and steel smelting industry

Babamotemi Oluwasola Itakorode; Raphael Emuebie Okonji; Odunayo Adedeji; Nkem Torimiro; Chinwe Onwudiegwu; Ademakinwa Oluwaseyi

doi:10.5897/AJBR2018.1014

African Journal of
Biochemistry Research

Abbreviation: Afr. J. Biochem. Res.
Language: English
ISSN: 1996-0778
DOI: 10.5897/AJBR
Start Year: 2007
Published Articles: 425

Full Length Research Paper

Studies on some physicochemical properties of Rhodanese synthesized by Bacillus cereus isolated from the effluents of iron and steel smelting industry

Babamotemi Oluwasola Itakorode

Babamotemi Oluwasola Itakorode
Department of Chemical Sciences, College of Natural and Applied Sciences, Oduduwa University Ipetumodu Ile-Ife, Osun State, Nigeria.
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Raphael Emuebie Okonji

Raphael Emuebie Okonji
Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Odunayo Adedeji

Odunayo Adedeji
Department of Biochemistry, Faculty of Science, Federal University Oye Ekiti, Ekiti State, Nigeria.
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Nkem Torimiro

Nkem Torimiro
Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Chinwe Onwudiegwu

Chinwe Onwudiegwu
African Institute for Science Policy and Innovation, Faculty of Technology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Ademakinwa Oluwaseyi

Ademakinwa Oluwaseyi
Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Article Number - 7B645D459927
Vol.13(1), pp. 1-8 , January 2019
https://doi.org/10.5897/AJBR2018.1014

Received: 22 October 2018
Accepted: 28 December 2018
Published: 31 January 2019

Abstract

The study purified and investigated the physicochemical properties of rhodanese (a cyanide detoxifying enzyme) synthesized by Bacillus cereus. This was with a view to producing an industrially important enzyme. The bacterial strain was identified as B. cereus by sequencing of its 16SrRNA gene. B. cereus rhodanese was purified with a fold of 3.53, yield of 36.80% and specific activity of 25.30 µmol/min/mg protein. The molecular weight determined on SDS-PAGE was 33.800 kDa. The enzyme exhibited maximum activity at 9.0 pH and 50°C. The K_ms of B. cereus rhodanese for sodium thiosulphate and potassium cyanide were 19.9 ± 1.05 and 31.4 ± 1.55 mM respectively, while V_max were 6.19 ± 0.40 and 4.83 ± 0.93 RU/ml respectively. The substrate specificity study using different sulphur compounds showed that the enzyme prefers sodium thiosulphate. The enzyme showed stability at a temperature range of 40-50°C. At 10 mM concentration, metals such as (BaCl₂, CaCl₂, MnCl_2,and SnCl₂) had little influence on the enzyme activity while NaCl and HgCl₂inhibited enzyme activity. The presence and biochemical properties of B. cereus rhodanese suggest its possible application in cyanide detoxification.