African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 383

Full Length Research Paper

Problems of interpretation of serum electrophoresis related to the manual phase during its realization with a semi-automatic automaton

Raoul Karfo
  • Raoul Karfo
  • Service of the Central Laboratory of Biochemistry, University Hospital Center Ibn Sina, Morocco.
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Elie Kabré
  • Elie Kabré
  • Training and Research Unit in Health Sciences, Ouaga University I Pr Joseph Ki-Zerbo, Burkina Faso.
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Nadia Safir
  • Nadia Safir
  • Service of the Central Laboratory of Biochemistry, University Hospital Center Ibn Sina, Morocco.
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Mounya Bouabdellah
  • Mounya Bouabdellah
  • Service of the Central Laboratory of Biochemistry, University Hospital Center Ibn Sina, Morocco.
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Laila Benchekroun
  • Laila Benchekroun
  • Service of the Central Laboratory of Biochemistry, University Hospital Center Ibn Sina, Morocco.
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Jean Sakandé
  • Jean Sakandé
  • Training and Research Unit in Health Sciences, Ouaga University I Pr Joseph Ki-Zerbo, Burkina Faso.
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Layachi Chabraoui
  • Layachi Chabraoui
  • Service of the Central Laboratory of Biochemistry, University Hospital Center Ibn Sina, Morocco.
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  •  Received: 25 November 2018
  •  Accepted: 02 April 2019
  •  Published: 30 April 2019

Abstract

Manual steps including preparation of samples and gel, and launching of automatic sequences can cause many difficulties in the interpretation of electrophoresis. Three observations of electrophoretic profiles on Hydragel 15β1-β2 agarose gel media led us to look for interference in the manual step of the technique used. After reconsidering all the steps of the manipulation and carefully observing the profiles obtained on the three plates of the agarose gel, the management of the deposition of the filter paper was identified as the cause in the first observation. The holding of the gel plate and the insufficient deposition of serum were identified as the main causes in the second observation. Mismanagement of the plate was identified as the cause in the third observation. All of these assumptions were confirmed by tests done on the bench. These observations remind us of the importance of the manual phase with semi-automatic devices for obtaining reliable results and for easy interpretation of  results.

 

Key words: Electrophoresis, migration, albumin, gamma, densitometer.