African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Purification and biochemical properties of lipase from raphia palm fruit mesocarp

T. Okunwaye
  • T. Okunwaye
  • Biochemistry Division, Nigerian Institute for Oil Palm Research, P.M.B. 1030, Benin City, Edo State, Nigeria.
  • Google Scholar
J.U. Obibuzor
  • J.U. Obibuzor
  • Biochemistry Division, Nigerian Institute for Oil Palm Research, P.M.B. 1030, Benin City, Edo State, Nigeria.
  • Google Scholar
E.A. Okogbenin
  • E.A. Okogbenin
  • Biochemistry Division, Nigerian Institute for Oil Palm Research, P.M.B. 1030, Benin City, Edo State, Nigeria.
  • Google Scholar


  •  Received: 15 January 2015
  •  Accepted: 11 May 2015
  •  Published: 30 May 2015

References

Abigor DR, Opute FI, Opoku AR, Osagie AU (1985). Partial purification and some properties of the lipase present in oil palm (Elaeis gunenis Jacq.) mesocarp. J. Sci. Food Agric. 36:599-605.
Crossref

 

Abigor RD (2002). Production of biodiesel fuel from vegetable oils using lipases from Pseudomonas cepacia and Jatropha curcas L. Ph.D. Thesis, University of Benin, Benin city, Nigeria. p.234.

 

 

Abigor RD, Uadia, PO, Foglia, TA, Haas, MJ, Scott K and Savary, BJ (2003). Partial purification and properties of lipase from germinating seeds of Jatropha cucas. J. Am. Oil Chem. Soc. 79(11):1123-1128
Crossref

 

 

Boland W, Frobl C, Lorenz M (1991). Estrolytic and lipolytic enzymes in organic synthesis. Synthesis 12:1049-1072.
Crossref

 

 

Doonan S (1996). Protein purification protocols. Method Mol. Biol. 59:135-140.
Crossref

 

 

Enujiugha VN, Thani FA, Sanni JM and Abigor RD (2004). Lipase activity in dormant seeds of African oil bean (Pentaclethra macrophylla Benth). Food Chem. 88(3):405-408.
Crossref

 

 

Forgia TA, Conkerton EJ, Philip E (1995). Regio selective analysis of triacylglycerols by lipase enzymes with attractive applications for stepwise hydrolysis of triolein using Candida rugosa lipase in biphasic oil-water system. Angew. Chem. Int. Ed. 37:1608-1633.

 

 

Freire GDM, Castilho FL (2008). Lipases em Biocatalise in: Bon et al.(org). Enyimas em biotechnologia: Producao Applicacao e Mercado, Rio d Janeiro, Interciencia. 1:369-385.

 

 

Gandhi NN (1997). Applications of lipase. J. Am Oil Chem. Soc. 74:621-634.
Crossref

 

 

Guit RP, Kloosterman M, Menderma GW, Mayer M, Meijer EM (1991). Lipase kinetics: Hydrolysis of triacetin by lipase from Candida cylindracea in a hollow-fiber membrane reactor. Biotechnol. Bioeng. 38:727-732
Crossref

 

 

Henderson J, Osborne DJ (1991). Lipase activity in ripening and mature fruit of the oil palm. Stability in vivo and in vitro. Phytochemistry 30:1073-1078.
Crossref

 

 

Hermansyah H, Kubo M, Shibasaki-Kitakawa N, Yonemoto T (2006). Mathematical model Humicola lanuginosa lipase on its thermal stability. J. Biochem. Biophys. Acta 1547:329-338.

 

 

Ibrahim CO (1996). Lipolytic enzyme technology for the palm oil industry proceedings of 1996 PORIM International Oil Congress-Chemistry, Technology, Soap and Detergent Industry Conference, 23-28 Sept., 1996 at hotel Isana, Kuala Lumpur, Malaysia. pp.133-149.

 

 

Iftikhar T, Niaz M, ZiaMA, Haq IU (2010c). Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor. Braz. J. Microbiol. 41(4):1124-1132.
Crossref

 

 

Khan MY, Dahot MV and Noomrio M H (1991). Investigation of lipase activity from Cajanus cajan L seed pack. J. Sci. Ind. Res. 34:384-386.

 

 

Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
Crossref

 

 

Lineweaver H, Burk D (1934). "The determination of enzyme dissociation constants". J. Am. Chem. Soc. 56(3):658-666.
Crossref

 

 

Lotrakul P, Dharmsthiti S (1997). Lipase production by Aeromonassobria LP004 in a medium containing whey and soybean meal. World. J. Mic. Biotechnol.13:163-166.
Crossref

 

 

Lotschert W, Beese G (1983). Collins Guide to Tropical Plants. London: Collins. ISBN 978-0-00-219112-8. 83-1496.

 

 

Lowry O, Rosebrough NJ, Farr AL (1951). Folin- phenol reagent. J. Biol. Chem. 193:265-275
PMid:14907713

 

 

Monnet TY, Pamphile KBK, Soro RY, Due EA, KouameLP (2012). Biochemical characterization of lipase activities in dormant seeds of T. catappa. J. Appl. Biosci. 24:337-3382.

 

 

Ohnishi K, Yoshida Y, Toita J and Sekiguchi J (1994). Purification and characterization of a novel lipolytic enzyme from Aspergillus oryzae. J. Ferm. Bioeng. 78(6):413-419.
Crossref

 

 

Pancreac'h G and Baratti JC (1996). Hydrolysis of p-nitrophenolpamitate in n-heptane by Pseudomonas cepacia lipase: a simple test for the determination of lipase activity in organic media. Enzyme Microb. Technol. 18:412-422.

 

 

Rahman R, Baharum SN, Basri M, Salleh AB(2005). High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5. Anal. Biochem. 341(2):267-274.
Crossref

 

 

Ratna AS (2008). Partial characterization of llipase from cocoa beans (Theobroma cacao. L.) of clone PBC 159. Ind. J. Chem. 8(3):448-453.