African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

A microassay for quantification of 2,2-diphenyl-1-picrylhydracyl (DPPH) free radical scavenging

Norma P. Meléndez
  • Norma P. Meléndez
  • Food Resarch Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, México.
  • Google Scholar
Virginia Nevárez-Moorillón
  • Virginia Nevárez-Moorillón
  • School of Chemistry, Universidad Autónoma de Chihuahua, Chihuahua, México.
  • Google Scholar
Raúl Rodríguez-Herrera
  • Raúl Rodríguez-Herrera
  • Food Resarch Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, México.
  • Google Scholar
José C. Espinoza
  • José C. Espinoza
  • School of Chemistry, Universidad Autónoma de Chihuahua, Chihuahua, México.
  • Google Scholar
Cristóbal N. Aguilar
  • Cristóbal N. Aguilar
  • School of Chemistry, Universidad Autónoma de Chihuahua, Chihuahua, México.
  • Google Scholar


  •  Accepted: 07 January 2014
  •  Published: 31 January 2014

References

Amatatongchai M, Laosing S, Chailapakul O, Nacapricha D (2012). Simple flow injection for screening of total antioxidant capacity by amperometric detection of DPPH radical on carbon nanotube modified-glassy carbon electrode. Talanta 97:267-272.
Crossref
 
Araujo P (2009). Key aspects of analytical method validation and linearity evaluation. J. Chromatogr. B. 877: 2224-2234.
Crossref
 
Bendini A, Cerretani L, Pizzolante L, Toschi TG, Guzzo F, Ceoldo S, Marconi AM, Andreetta F, Levi M (2006). Phenol content related to antioxidant and antimicrobial activities of Passiflora spp. extracts. Eur. Food Res. Technol. 223:102-109.
Crossref
 
Ciesla L, Kryszen J, Stochmal A, Oleszeck W, Waksmundzka-Hajnos M (2012). Approach to develop a standardized TLC-DPPH test for assessing free radical scavenging properties of selected phenolic compounds. J. Pharma. Biomed. Anal. 70: 126-135.
Crossref
 
Daker M, Abdullah N, Vikineswary S, Goh PC, Kuppusamy UR (2008). Antioxidant from maize and maize fermented by Marasmiellus sp. as stabilizer of lipid-rich foods. Food Chem. 107:1092-1098.
 
Dawidowicz AL, Wianowska D, Olszowy M (2012). On practical problems in estimation of antioxidant activity of compounds by DPPH method (problems in estimation of antioxidant activity). Food Chem. 131:1037-1043.
Crossref
 
Fernandez-Orozco R, Roca M, Gandul-Rojas B, Gallardo-Guerrero L (2011). DPPH-scavening of chloroplastic pigments and phenolic compounds of olive fruits (cv. Arbequina) during ripening. J. Food Compos. Anal. 24:858-864.
Crossref
 
Hibbert DB (1999). Method validation of modern analytical techniques. Accred. Qual. Assur. 4:352-356.
Crossref
 
Huang D, Ou B, Prior RL (2005). The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53:1841-1856.
Crossref
 
Locatelli M, Gindro R, Travaglia F, Coïson JD, Rinaldi M, Arlorio M (2009). Study of the DPPH-scavenging activity: Development of a free for the correct interpretation of data. Food Chem. 114:889-897.
Crossref
 
Mishra K, Ojha H, Chaudhury NK (2012). Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chem. 130:1036-1043.
Crossref
 
Molyneux P (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26(2):211-219.
 
Osman AM (2011). Multiple pathways of the reactions of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) with (+)-catechin: Evidence for the formation of a covalent adduct between DPPH and the oxidized form of the polyphenol. Biochem. Biophys. Res. Commun. 412:473-478.
Crossref
 
Sharma OP, Bhat TK (2009). DPPH antioxidant assay revisited. Food Chem.113:1202-1205.
Crossref
 
Sreejalekshmi KG, Krishnan KA (2011). In: Sabu A, Roussos S, Aguilar CN (eds) Chemistry and biotechnology of polyphenols. CiBET Publishers, India
 
Swapna TS, Sabu A (2011). In: Sabu A, Roussos S, Aguilar CN (eds) Chemistry and biotechnology of polyphenols. CiBET Publishers, India.
 
Szabo MR, Iditoiu C, Chambre D, Lupea AX (2007). Improved DPPH determination of antioxidant activity spectrophotometric assay. Chem. Pap. 61(3):214-216.
Crossref
 
Uriarte-Pueyo I, Calvo MI (2009). Assay conditions and validation of a new UV spectrophotometric method using micro-plates for the determination of polyphenol content. Fitoterapia 80:465-467.
Crossref
 
Wang X, Germansderfer A, Harms J, Rathore AS (2007). Using statistical analysis for setting process validation acceptance criteria for biotech products. Biotechnol. Prog. 23:55-60.
Crossref