African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5177

Full Length Research Paper

Isolation and polyphasic characterization of aerobic actinomycetes genera and species rarely encountered in clinical specimens

  Habiba Zerizer1,4*, Bernard La Scolat2, Didier Raoult2, Mokhtar Dalichaouche3 and Abderrahmen Boulahrouf4
  1Institut de la Nutrition et des Technologies Agro-Alimentaires (INATAA), Université 1 de Constantine, Algérie. 2Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, Université de la Méditerranée, Marseille, France. 3Service des Maladies Infectieuses, Hôpital Universitaire de Constantine, Algérie. 4Laboratoire de Génie Microbiologique et Applications, Université Mentouri de Constantine, Algérie.
Email: [email protected]

  •  Accepted: 08 July 2013
  •  Published: 12 August 2013

Abstract

 

The aim of this study was to identify aerobic actinomycetes strains belonging to genera and species rarely encountered in infections. Clinical specimens (sputum, gastric fluid and abscess pus) are collected from patients with symptoms of tuberculosis, pneumopathy, septicemy or having abscess, hospitalized in different services of infectious diseases in Constantine University Hospital Center, East of Algeria. A total of 49 strains of aerobic actinomycetes were isolated; among which 40 ones belong toStreptomyces, Nocardia and Actinomadura genera; however, nine strains are members of other actinomycetes genera, characterized in this study. Phenotypic, chemotaxonomic proprieties and sequencing 16S rRNA gene established identification of these isolates to:Nonomuraea roseolaKribbella sp., Micromonospora aurantiacaNocardiopsissynnemataformans and Saccharothrix longispora. Identified isolates belonged to genera and species rarely encountered in clinical specimens; their role in pathology must be defined.

 

Key words: Uncommon aerobic actinomycetes, clinical specimens, polyphasic taxonomy.