African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5172

Table of Content: 12 July, 2013; 7(28)

July 2013

Type E botulism associated with crude beef: A case report

  Botulism remains infrequent but is widely feared because it can be severe and is potentially lethal. Foodborne botulism results from contaminated foodstuffs in whichClostridium botulinum spores have been allowed to germinate in anaerobic conditions. This typically occurs in home-canned food substances and fermented uncooked dishes. We present an outbreak of type E botulism in China. The diagnosis was...

Author(s):   Jiu-Cong Zhang#, Fang Xie# and Li Sun

July 2013

Properties of Enterococcus thailandicus isolates from beavers

  Enterococcus thailandicus is characterized as the species of the genus Enterococcus, Family Enterococcacae, Order Lactobacillales, Class Bacilli, Phylum Firmicutes. This species novum is catalase-negative, chain forming coccus, and first detected from the fermented sausage (mum) in Thailand. Up to now, occurrence of enterococcal species from many animal sources was detected. Herbivor Castor...

Author(s):   A. Lauková, A. Kandričáková, J. Imrichová, V. Strompfová, R. Miltko, B. Kowalik and G. Belzecki

July 2013

Effect of ambient storage on the microbial characteristics of traditional dried anchovies (Encrasicholina punctifer)

  The aim of this study was to evaluate the safety of traditional dried anchovies by characterizing their microbial flora. Dried anchovies were stored at ambient temperature for three months and total aerobic bacterial (TAB), Staphylococcus aureus, Enterobacteriaceae, histidine decarboxylating bacterial (HDB), lysine decarboxylating bacterial (LDB) and ornithine decarboxylating bacterial (ODB) counts were...

Author(s):   Ismail M. Al Bulushi, Nejib Guizani and Gary A. Dykes

July 2013

Biofilm, protease and lipase properties and antibiotic resistance profiles of staphylococci isolated from various foods

  In this study, a total of 209 isolates were isolated from raw calf meat (minced), chicken drumsticks, raw milk, ice cream and white cheese samples. One hundred and seventy-five (83.7 %) coagulase-negative Staphylococci (CNS) and 34 (16.3%) coagulase-positive staphylococci (CPS) were isolated from these samples. The majority of Staphylococcusisolates showed biofilm formation (75.1%) and slime...

Author(s):   Neslihan Gundogan and Ozlem Ataol

July 2013

A modified staining technique for the anatomical observation of mycorrhizal roots of woody trees

  Modifications to the root staining method described by Phillips and Hayman (1970) were performed in order to develop a clear anatomical visualization of the mycorrhization status in avocado roots. Diverse KOH and H2O2 concentrations and various incubation times during clearing and acidification steps were assayed. The best results were obtained with the following treatment: once dried at 50°C for 12...

Author(s):   Ana Tztzqui Chávez-Bárcenas, Judith Lua-Aldama, Isaac Alejandro Salmeron-Santiago, María Blanca Silva-Adame, Pedro Antonio García-Saucedo and Victor Olalde-Portugal

July 2013

Influence of straw degrading microbial compound on wheat straw decomposition and soil biological properties

  The main challenge to successful use of wheat straw as soil amendment is its slow decomposition rate as compared to other crop residues. In order to solve this problem and increase farmers’ acceptability, inoculation of straw amended soil with microbial compounds to hasten decomposition is being promoted in China. In this experiment, the effect of three types of industrially produced composite microbial...

Author(s):   Ogunniyi Jumoke Esther, Tian Xiao Hong and Guo Chun Hui

July 2013

Bacterial community composition of a saline-alkaline soil amended or not with wastewater sludge and contaminated with polycyclic aromatic hydrocarbons (PAHs)

  The purpose of this study was to determinate the dynamics of the bacterial community composition, that is, diversity and abundance of microbial soil communities through PCR-DGGE of 16S rDNA gene fragments from a saline-alkaline soil. The soil and/or wastewater sludge were contaminated or not with phenanthrene and anthracene and spiked or not with acetone or polycyclic aromatic hydrocarbons (PAHs)-solution...

Author(s):   Fabián Fernández-Luqueño, Edgar Vázquez-Núñez, Francisco J. Zavala-Díaz de la Serna, Conrado Martínez-Suárez, Gabriela Salomón-Hernández, Cesar Valenzuela-Encinas, Olivia Franco Hernández, Juan Manuel Ceballos-Ramírez and Luc Dendooven

July 2013

Comparative effectiveness of different isolates of Azospirillum on nitrogen fixation and yield and yield attributing characters of tomato in Chhattisgarh

  Among the total six isolates, isolates LAIS-21, LAIK-06 and LAIJ-04 were more potential regarding nitrogen fixation and fixed 19.2 mg, 14.9 mg and 11.0 mg N g-1 malate, respectively. Out of these three isolates, isolate LAIS-21 recorded higher plant height (45.5 cm), number of fruits per plant (36.2), fruit diameter (4.8 cm), fruit weight (57.9), root length (10.3 cm), fresh root weight (23.7 g), yield...

Author(s):   Tripathi, J., Singh, A. K., Tiwari, P. and Menaka M

July 2013

Antimicrobial activity of aqueous extracts of sea cucumber (Isostichopus badionotus) from the coast of Yucatan, Mexico

  The antibacterial activity of aqueous extracts from different tissues of Isostichopus badionotus was evaluated in this study. The antibacterial activity was detected in aqueous extracts of muscle and respiratory tract. A high antibacterial activity was found in the Membrane Protein fraction (MP) muscle...

Author(s):   Fernando Moguel-Salazar, Elizabeth Ortiz-Vázquez, Rossanna Rodríguez-Canuland Leticia Olivera-Castillo

July 2013

Antimicrobial susceptibility pattern of bacteria isolated from burn wounds in a tertiary care hospital in Pakistan

  Burns constitute a major health concern with respect to morbidity and mortality; due to the risk of burn wounds infections caused by multi-drug resistant nosocomial pathogens. The present study was carried out to recover, identify the isolates, and to determine the antimicrobial susceptibility patterns of these pathogens from burn wounds in Khyber Teaching Hospital (KTH) Peshawar, during September 2007 to...

Author(s):   Jawad Ahmed, Amjad Ali, Masroor Hussain and Habib Ahmad

July 2013

Degradation kinetics of resorcinol by Enterobacter cloacae isolate

  Resorcinol was utilized as the sole carbon and energy source by Enterobacter cloacae(identification by 16S rDNA nucleotide sequencing Genbank Accession Number JN093148). The different concentration of resorcinol utilized by the bacterial isolate ranged between 55 and 220 mg l-1 at 30°C and pH of 7.0. It was observed that the batch experimental results were best fitted for Michaelis-Menten...

Author(s):   Marissa R. Jablonski, Shraddha Shaligram, Asifa Qureshi, Hemant Purohit and John R. Reisel  

July 2013

Characterization of six phosphate-dissolving bacteria isolated from rhizospheric soils in Mali

  Maize rhizospheric soils in Mali were analysed for their content in microorganisms capable of dissolving phosphate rock and producing plant growth substances. Six bacteria were isolated and found to have the capacity to dissolve /solubilize the Tilemsi phosphate rock (TPR) by producing low molecular weight organic acids and phosphorus cycle enzymes. The bacterial isolates had an average of...

Author(s):   Lamine Traoré, Cindy H. Nakatsu, Allysa DeLeon and Diane E. Stott

July 2013

Antimicrobial properties of the bacterial associates of the Arctic lichen Stereocaulon sp

  Four bacterial associates were isolated from the Arctic lichen Stereocaulon sp. and identified based on their complete 16S rRNA gene sequences. Cell cultures were extracted using different solvents and tested for their antimicrobial properties using paper disk diffusion assay and MIC test against six test bacteria. Results show that the ethyl acetate of bacterial isolate is a most effective solvent...

Author(s):   Mi-Kyeong Kim, Hyun Park and Tae-Jin Oh

July 2013

Identification of four major toxins of Clostridium perfringens recovered from clinical specimens

  A simple polymerase chain reaction (PCR) procedure was used to identify four toxitypes of Clostridium perfringens collected from different origins. Eighteen (18) strains of C. perfringens were identified and typed by classical methods (dermonecrotic method in guinea pigs and sero-neutralization test in mice). All the strains were analyzed by PCR using gene of toxin alpha, gene of...

Author(s):   Ashgan M. H., Abdullah A. Al-Arfaj and Moussa, I. M.

July 2013

Development of a cell line from the gill of koi and molecular characterization of KHV isolated in Taiwan

A new cell line (KoG) was developed from the gill of koi, Cyprinus carpio. KoG cells were composed of epithelial-like cells and fibroblast-like cells. The cells were maintained well at 25°C in Leibovitz L15 medium supplemented with 10% fetal calf serum. Chromosome analysis showed that the KoG cells had 43 diploid chromosomes at passage 40. The KoG cells were used to test the susceptibility of a koi...

Author(s): Chien Tu, Chia-Yu Hsieh, Shao-Kuang Chang, Je-Fu Su and Meei-Mei Chen

July 2013

Effect of added nopal cactus (Opuntia ficus-indica) on microbial content in raw milk

  Nopal cactus (Opuntia ficus-indica) represents an alternative for cattle feed during the drought season in the drier regions of Mexico. Previously, we have shown that feed with the addition of nopal reduced aerobic mesophiles (AM) and coliform (C) counts in milk. In order to determine whether the nopal additive has some direct effect on the microbiological quality of milk, nopal mucilage (NM), nopal epidermis...

Author(s):   Ortiz-Rodríguez R. Valdez-Alarcón J. J., Garcidueñas-Piña R., Chávez-Moctezuma M. P., Val-Arreola D., Hernandez Valdes E. F. and Pérez–Sánchez R. E.

August 2013

Isolation and polyphasic characterization of aerobic actinomycetes genera and species rarely encountered in clinical specimens

  The aim of this study was to identify aerobic actinomycetes strains belonging to genera and species rarely encountered in infections. Clinical specimens (sputum, gastric fluid and abscess pus) are collected from patients with symptoms of tuberculosis, pneumopathy, septicemy or having abscess, hospitalized in different services of infectious diseases in Constantine University Hospital Center, East of Algeria....

Author(s):   Habiba Zerizer, Bernard La Scolat, Didier Raoult, Mokhtar Dalichaouche and Abderrahmen Boulahrouf

July 2013

The role of ferrates (VI) as a disinfectant: Quantitative and qualitative evaluation for the inactivation of pathogenic bacteria

  Iron compounds in the oxidation state (VI) have the advantage of being powerful antioxidants and bactericides, which explains their particular interest in water treatment.The aim of this work was to investigate the ability of inhibition of pathogenic microorganisms (Escherichia coli, Salmonella, Staphylococcus aureus, Bacillus sp., Pseudomonas sp., Enterococcus feacalis) by ferrate and for the...

Author(s):   Abdellatif EL MAGHRAOUI, Abdelaziz ZEROUALE, Mustapha IJJAALI and Kawtar FIKRI BENBRAHIM

July 2013

Comparison of OSOM BV Blue test with conventional methods for diagnosis of bacterial vaginosis

  Bacterial vaginosis is the commonest cause of vaginal discharge in sexually active females. It is often associated with adverse pregnancy outcomes and increased susceptibility to other sexually transmitted diseases. In the present study, we evaluated efficacy of OSOM BV Blue test and compared it with conventional methods like Gram staining and Amsel’s criteria for diagnosing bacterial vaginosis. A total...

Author(s):   Razia Khatoon, Noor Jahan, Siraj Ahmad, and Tamkin Rabbani

July 2013

Estimation of microbial flora in mince meat collected from Nandus nandus, Channa marulius and Puntius sarana during six months storage at -20°C

  The study was designed to investigate the microbial estimation in the fish mince collected from Nandus nandus, Channa marulius and Puntius sarana which was stored at -20°C for a period of six months and analyzed every fortnightly for total plate count. During the study period, in the mince of N. nandus, C. marulius and P. sarana, there was a continuous decrease...

Author(s):   Ritu Agrawal, Malobica Das, A. K. Upadhyay and Mohd. Danish