African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Generation of green fluorescent protein vector transformed Paecilomyces lilacinus Strains

  Xiaoli Liu1, 2, Xizhuo Wang2, Laifa Wang2, Qinglong Shu1, Zhihua Cao1  
1School of Forest and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China. 2Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.
Email: [email protected]

  •  Accepted: 08 March 2013
  •  Published: 26 March 2013

Abstract

Paecilomyces lilacinus is one of the most important biocontrol fungi and has been applied extensively for controlling root-knot nematodes. The protoplast/PEG/CaCl2 method was used to generate green fluorescent protein (GFP) transformants of two strains ofPaecilomyces lilacinus (strain 20-7 and 618). Two transformed strains were successfully generated and identified by fluorescence microscopy. Transformation was confirmed by recovery, cloning and sequencing of polymerase chain reaction (PCR) products and Southern blot analysis. Two stably transformed strains were isolated (20-7 and 618 strain). GFP transformants of Paecilomyces lilacinus provide useful resources for elucidating the mechanism of controlling root-knot nematodes.

 

Key words: Paecilomyces lilacinus, green fluorescent protein, fluorescence expression, transformants.