March 2013
Medium optimization for the production of a metagenome-derived β-galactosidase by Pichia pastoris using response surface methodology
To investigate the optimal medium for the production of a metagenome-derived β-galactosidase (ZQ114) by Pichia pastorisi, Plackett and Burman design was applied to screen the significance of eight medium compositions, and three factors (methanol, sorbitol and MgSO4.7H2O) were found to have significant effect on the production of ZQ114. Then, the steepest ascent method was further used to determine their...
March 2013
Effects of salt, sugar and starter culture on fermentation and sensory properties in Shidal (a fermented fish product)
There are several traditional fermented fish products in Northeast India and shidal is one of them. It is made from small carps (Puntius spp.) with a maturation period of 4 to 6 months. The traditional method of shidal preparation varies from place to place. In this study traditional methods of Assam and Tripura were studied and standardized. The traditional shidal was collected in...
March 2013
Production of polyunsaturated fatty acid (DHA) by mangrove-derived Aplanochytrium sp.
This study optimized the production of docosahexaenoic acid (DHA) by Aplanochytriumsp. The microbe was cultured under different conditions, which were optimized by using the central composite design model of the response surface methodology (RSM). The interaction and individual effects of important factors for DHA production byAplanochytrium sp., was analysed. DHA production was attained at the...
March 2013
Molecular detection of invA, ssaP, sseC and pipB genes in Salmonella typhimurium isolated from human and poultry in Iran
Infections due to Salmonella serovars are a great risk to public health. It is known that genes involved in pathogenesis of Salmonella serovars are clustered within Salmonellapathogenicity Islands. We have characterized four genes (invA, ssaP, sseC, and pipB) coded by 3 pathogenicity Islands among 54 Salmonella typhimurium isolated from poultry samples in Iran. A...
March 2013
The pattern of autoantibodies related to false negative results of serological tests in the diagnosis of human brucellosis
For the diagnosis of human brucellosis it is essential to perform diagnostic tests because of the wide variety of clinical manifestations of the disease. Serological tests are widely used for the diagnosis of brucellosis. However, blocking substances limit the use of standard tube agglutination test. Two hundred and forty two (242) serum samples were collected from healthy persons and persons in high-risk occupation....
March 2013
Generation of green fluorescent protein vector transformed Paecilomyces lilacinus Strains
Paecilomyces lilacinus is one of the most important biocontrol fungi and has been applied extensively for controlling root-knot nematodes. The protoplast/PEG/CaCl2 method was used to generate green fluorescent protein (GFP) transformants of two strains ofPaecilomyces lilacinus (strain 20-7 and 618). Two transformed strains were successfully generated and identified by fluorescence microscopy....
March 2013
Ethanol production from deproteinized cheese whey fermentations by co-cultures of Kluyveromyces marxianus and Saccharomyces cerevisiae
An application of the co-culture of Kluyveromyces marxianus and Saccharomyces cerevisiae for ethanol production from deproteinized cheese whey was established. Among several co-cultures examined, the co-culture of S. cerevisiae UFLA KFG33(ethanol over-producer) and K. marxianus (UFLA KF22) showed the highest value of ethanol production (16.02±0.11 g L-1) and...
March 2013
The occurrence of pink mold rot fungus Trichothecium roseum on tomatoes in Korea
Trichothecium roseum DUCC502 was isolated from leaves of tomato plants at a greenhouse located in Buyeo, Chungchungnamdo. The colony color of the isolate was white initially and became pale pink on potato dextrose agar and oatmeal agar. Conidiophores of the isolate were long and slender, unbranched, and 73 – 112 x 2.1 – 3.3 µm in size. Its conidia were two-celled, hyaline...
March 2013
Effect of thyme essential oil and packaging treatments on chemical and microbiological properties of fresh rainbow trout (Oncorhynchus mykiss) fillets during storage at refrigerator temperatures
In this research, the effect of thyme essential oil (EO) and packaging treatments (vacuum and modified atmosphere) on fresh rainbow trout fillets during storage for a period of 18 days at 4°C was investigated. Fillets were subjected to microbiological (total viable count(TVC), Pseudomonas, lactic acid bacteria, Enterobacteriaceae) and chemical [pH,thiobarbituric acid reactive...
March 2013
Production and partial purification of pectin lyase by Aspergillus niger grown on orange peels
The peel of citrus fruits contains a large percentage of pectin which can be a good substrate for pectinolytic microorganisms. These microbes secrete large amount of extracellular enzymes to degrade the cell wall of substrates. The current study was conducted for the production and characterization of pectin lyase from Aspergillus nigerusing citrus fruits peel as a substrate. The optimum pectin lyase production was...
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