African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Effectiveness of Myrothecium roridum for controlling water hyacinth and species identification based on molecular data

Orawan Piyaboon
  • Orawan Piyaboon
  • Bioproducts Science, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand
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Arm Unartngam
  • Arm Unartngam
  • Bioproducts Science, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand
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Jintana Unartngam*
  • Jintana Unartngam*
  • Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand
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  •  Received: 26 July 2013
  •  Accepted: 26 February 2014
  •  Published: 26 March 2014

Abstract

Myrothecium roridum isolates were evaluated for their effectiveness in controlling the aggressive water hyacinth and molecular identification was conducted using internal transcribed spacer (ITS) rDNA region and amplified fragment length polymorphism (AFLP) markers. M. roridum isolates were collected from nine provinces of Thailand. Seventy isolates were included in the primary screening, using colonial growth rate and spore number measurement. The results indicate that five isolates were the most aggressive and so were selected to evaluate their effectiveness in controlling the water hyacinth. The result showed that M. roridum isolate KKFC 408 had significantly highest disease severity (P<0.05) and the highest reduction for the fresh weight of water hyacinth. These five isolates DNA were amplified and sequenced using ITS1 and ITS4 universal primer for species identification. The data analyzed showed that five sequences of M. roridum were in the same group agreeing with other sequences of M. roridum recorded in the GenBank database, which correlated to morphological observation results. The AFLP result indicated that fifteen isolates of M. roridum were divided into four subgroups which were not correlated to the geographical area.

 

Key words: Amplified fragment length polymorphism (AFLP), internal transcribed spacer (ITS) of rDNA region, mycoherbicide.

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