March 2014
Phylogenetic analysis of genotype VII of new castle disease virus in Indonesia
Newcastle disease (ND) is a very contagious disease in chickens and turkeys and one of the most important diseases of poultry in the world. The infection causes sudden death with high mortality. In Indonesia, Newcastle disease in recent years showed symptoms slightly different from previous symptoms of this disease. NDV infection of genotipe VII has been reported to cause this outbreak in several commercial poultry...
March 2014
The associated protein screening and identification in Proteus mirabilis swarm colony development
Proteus mirabilis colonies exhibit striking geometric regularity, and swarm colony terraces correspond to one swarming-plus-consolidation cycle. Basic microbiological methods and imaging techniques were used to measure periodic macroscopic events in swarm colony morphogenesis. Here, adding a respiratory enzyme indicator, 2,3,5-triphenyltetra-zolium chloride (TTC) into the culture medium, the bacteria on the rings...
March 2014
Characterization of invertase from Saccharomyces cerevisiae MTCC 170
Activity and stability of invertase obtained from Saccharomyces cerevisiae MTCC 170 were characterized with parameters like pH, temperature, metal ions, surfactants and chemical inhibitors. The pH stability of this enzyme was observed between pH 2 and 9 with an average of 63% retaining activity for 24 h. The crude enzyme showed optimum activity at pH 6 and 30°C. Enzyme activity was increased in the presence of 5 mM...
March 2014
Involvement of clathrin and β-arrestins in Aggregatibacter actinomycetemcomitans endocytosis of human vascular endothelial cells
Phosphorylcholine (PC) has been reported to help PC-bearing Aggregatibacter actinomycetemcomitans to gain access to the general circulation via interaction with the platelet-activating factor receptor (PAFR) on endothelial cells. However, the underlying mechanism remains unclear. Here, we found that inhibition of clathrin and β-arrestins blocked bacterial PC binding to PAFR which signiï¬cantly attenuated A....
March 2014
Bio-efficacy of fungicides, bioagents and plant extracts/ botanicals against Alternaria carthami, the causal agent of Alternaria blight of Safflower (Carthamus tinctorius L.)
A total of eleven fungicides namely: mancozeb 75 WP, chlorothalonil 75 WP, copper oxychloride 50 WP, thiram 75 WP, captan 50 WP, difenconazole 25 EC, carbendazim 50 WP, hexaconazole 5 EC, propiconazole 25 EC, penconazole 10 EC and metalyxil + mancozeb (Ridomil) 68 WP were evaluated (at 500, 1000 and 1500 ppm each) in vitro against Alternaria carthami. All the fungicides tested caused significant inhibition at all three...
March 2014
Assessment of ants as bacterial vector in houses
Studies of arthropods have fundamental importance to identifying both ant species and microorganisms they carry. The objective of this study was to identify ant morph species found in residences, characterize the bacteria associated with the ants, assess bacterial resistance to antibiotics and analyze the plasmid profiles of the microorganisms found. The ant collections were carried out in the kitchens of 50 residences...
March 2014
Isolation and identification of probiotic lactic acid bacteria from curd and in vitro evaluation of its growth inhibition activities against pathogenic bacteria
The aim of this study was to isolate and identify probiotic lactic acid bacteria from curd and evaluate in vitro, its growth inhibition activities against pathogenic bacteria. A total of nine strains of Lactobacillus were isolated from curd and identification of strains was done by biochemical and physiological tests and Lactobacillus leichmannii, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus brevis,...
March 2014
Bacterial diversity in the rumen of mithun (Bos frontalis) fed on mixed tree leaves and rice straw based diet
This work was done to study the bacterial diversity of mithun (Bos frontalis) fed on mixed tree leaves and rice straw based diet. Genomic DNA was extracted from the rumen liquor of mithun, 16S rDNA sequences were amplified, cloned and randomly selected for sequencing. The nearest neighbors were retrieved from the NCBI through a BLAST search and a phylogenetic tree was constructed. In our findings, 12% of clones showed...
March 2014
Biofilm in aquaculture production
A biofilm is an assemblage of microbial cells which is irreversibly associated with a surface and enclosed in a matrix of primarily polysaccharide material. It may form on a wide variety of surfaces, including living tissues, medical devices, industrial or potable water system pipe or natural aquatic systems. A well-diversified organism such as algae, bacteria, protozoa, arthropods, etc. may be observed in the biofilm...
March 2014
Effectiveness of Myrothecium roridum for controlling water hyacinth and species identification based on molecular data
Myrothecium roridum isolates were evaluated for their effectiveness in controlling the aggressive water hyacinth and molecular identification was conducted using internal transcribed spacer (ITS) rDNA region and amplified fragment length polymorphism (AFLP) markers. M. roridum isolates were collected from nine provinces of Thailand. Seventy isolates were included in the primary screening, using colonial growth rate and...
March 2014
Bacterial pathogens associated with infectious diarrhea in King Khalid University Hospital, Riyadh Saudi Arabia 2005-2010
Diarrhoeal diseases are major cause of morbidity worldwide. In developed and developing countries, Salmonella, Shigella and Campylobacter species are the main bacterial causes. This study aimed to investigate the occurrence and antimicrobial susceptibility of bacteria causing diarrhea in Riyadh, during a six years period. This study was performed in Riyadh capitol of Saudi Arabia between January 2005 to December 2010....
March 2014
Detection of selected anaerobic pathogens in primary and secondary endodontic infections in a Turkish population
The aim of this investigation was to examine the presence of 8 bacterial anaerobic species in endodontic samples from patients with primary and secondary infection. The association of clinical signs and symptoms with constituent species were also evaluated. Microbial samples were obtained from 72 teeth with primary endodontic infection and 35 teeth with secondary endodontic infection. DNA was extracted from samples and...
Advertisement
Advertisement
