African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Screening of filamentous fungi from Brazilian rainforests for enzyme production

Erica Benjamim da Silva*
  • Erica Benjamim da Silva*
  • Food Science Department, School of Food Engineering, University of Campinas, 80 Rua Monteiro Lobato, postal Code 13083-862, Campinas-SP, Brazil
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Andre Ohara
  • Andre Ohara
  • Food Science Department, School of Food Engineering, University of Campinas, 80 Rua Monteiro Lobato, postal Code 13083-862, Campinas-SP, Brazil
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Cinthia Marques Fernandes
  • Cinthia Marques Fernandes
  • Food Science Department, School of Food Engineering, University of Campinas, 80 Rua Monteiro Lobato, postal Code 13083-862, Campinas-SP, Brazil
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Paula de Paula Menezes Barbosa
  • Paula de Paula Menezes Barbosa
  • Food Science Department, School of Food Engineering, University of Campinas, 80 Rua Monteiro Lobato, postal Code 13083-862, Campinas-SP, Brazil
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Gabriela Alves Macedo
  • Gabriela Alves Macedo
  • Food Science Department, School of Food Engineering, University of Campinas, 80 Rua Monteiro Lobato, postal Code 13083-862, Campinas-SP, Brazil
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  •  Received: 31 October 2014
  •  Accepted: 19 January 2015
  •  Published: 04 February 2015

Abstract

Screening of new microbial strains for enzyme production is a key step in the improvement of industrial bioprocesses. The objective of this work was to isolate fungal strains from Brazilian rainforests and to evaluate their potential to produce industrial enzymes by solid state fermentation. First, 131 strains of filamentous fungi were isolated from Amazon rainforest and Atlantic rainforest regions. Strains selected by simple plate assay had their ability to produce lipase and tannase by fermentation of wheat bran evaluated. Maximum enzymatic activity of 1.35 and 18.7 U/mL were reached by lipase and tannase, respectively. The strain IB28a was identified by molecular and morphologic techniques as Colletotrichum sp. Lipase produced by this strain reached specific activity of 25.97 U/mg and showed Km and Vmax of 6.3 and 19.5, respectively. Lipase from Colletotrichum sp. had an optimal temperature range of 25 to 35°C and optimal pH range of 6.5 to 7.0. Moreover, the enzyme was stable after 1 h at temperatures up to 40°C and after 24 h at pH 6.5.

Key words: Bioprospection, filamentous fungi, lipase, tannase.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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