African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Flagellin promotes propagation of pine wood nematode and its carrying Pseudomonas fluorescens GcM5-1A in callus of Pinus thunbergii through inducing cell death

Lei Zhang1, Tongqing Yue1, Boguang Zhao2, Daosen Guo1, Binwei Wu, Tongsong Wang1, Wei Jin1 and Ronggui Li1*
1Department of Biology, Qingdao University, Qingdao 266071, China. 2Department of Forest Entomology and Pathology, Nanjing Forest University, Nanjing 210037, China.
Email: [email protected]

  •  Accepted: 24 January 2012
  •  Published: 16 February 2012

Abstract

Pine wilt disease (PWD), which is caused by pine wood nematode (PWN),Bursaphelenchus xylophilus has become a widespread threat to forests. Recently, more and more researches indicate that bacteria associated with PWN play an important role in pine wilt. We previously found that flagellin secreted from Pseudomonas fluorescensGcM5-1A could cause the death of suspension cells of Pinus thunbergii in vitro, but the mechanism is not clear. In this paper, flagellin of P. fluorescens GcM5-1A was found to interact with suspension cells, which resulted in disintegration of nuclei and degradation of genomic DNA. Inoculation test indicated that population levels of PWN and P. fluorescensGcM5-1A inoculated on dead callus of P. thunbergii either pretreated with flagellin or caused by heat treatment were much larger than those inoculated on live callus, respectively. Furthermore, flagellin alone could also promote the propagation of PWN and its carrying P. fluorescens GcM5-1A. The results implied that toxins such as flagellin secreted by bacteria associated with PWN might participate in the pathogenesis of PWD by inducing host cell death and promoting propagation of the 2 pathogens PWN and bacteria it carries.

 

Key words: Flagellin, Pinus thunbergii, callus, propagation, pine wood nematode,Pseudomonas fluorescens GcM5-1A.