African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Combination of Dichelobacter nodosus LAMP with Fusobacterium necrophorum LAMP for detecting hoof swabs from dairy cattle footrot

Sun D. B., Wu R., Guo D. H.*, He X. J., Zheng J. S., Wang J. F., Lin Y. C., Han X., Wang Y. Q. and Guo T. T.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing High-tech Industrial Development Zone, Daqing 163319, P. R. China.
Email: [email protected]

  •  Accepted: 01 March 2011
  •  Published: 18 March 2011

Abstract

 

To investigate the presence of Fusobacterium necrophorum (F. necrophorum) and Dichelobacter nodosus (D. nodosus) in dairy cattle footrot, a loop-mediated isothermal amplification method for detection of D. nodosus (Dn-LAMP) was developed and evaluated using species-specific16S rRNA gene as target gene. The Dn-LAMP showed no cross reaction with several common pathogens from cattle, and detection limit of the Dn-LAMP was 10 cfu/mL of D. nodosus. Amplicons of the Dn-LAMP could be detected by visual inspection. 189 hoof swabs from footrot-infected dairy cows in Heilongjiang province of China were detected using Dn-LAMP and Fn-LAMP for diagnosis of F. necrophorum, respectively. In 189 hoof swabs, 9 were positive for D. nodosus, and 95 were positive forF. necrophorum. These data suggested that combination of Dn-LAMP with Fn-LAMP will have a potential use for etiology investigation of footrot in dairy cattle.

Key words: Dichelobacter nodosus, Fusobacterium necrophorum, LAMP.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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