African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Chemical composition, in vitro antifungal and antioxidant activities of essential oil from Cotula coronopifolia L. growing in Tunisia

Fethi Bel Hadj Kether1, Mohamed Ali Mahjoub1, Samia Ammar Mahjoub1, Karima Belhaj Salah3, Ahmed Noureddine Helal1 and Zine Mighri2*        
1Université de Monastir, Laboratoire de Génome, Diagnostic Immunitaire et Valorisation, (03/UR /09-01) Institut Supérieur de Biotechnologie de Monastir, 5000 Monastir, Tunisie. 2Université de Monastir, Faculté des sciences-Département de chimie. UR 1204-Chimie Appliquée-Environnement 5000 Monastir, Tunisie. 3Laboratoire des Maladies transmissibles et substances biologiquement actives, Faculté de pharmacie 5000 Monastir Tunisie.  
Email: [email protected]

  •  Accepted: 23 February 2011
  •  Published: 30 May 2012

Abstract

The chemical composition of Cotula coronopifolia L. essential oils obtained from the aerial parts (flowers, leaves, stems) and roots by hydro distillation was analyzed by gas chromatography/ mass spectrum (GC/MS). Fifty seven components were identified. The most important compound detected in leaves was Agarospirol (10.43%). Hexacosane was identified in flowers with an abundance percentage of (31.7%) and 1-Eicosanol (17.1%) in stem. The major constituents identified in the root oils were Heptacosane (28.4%), 1-Eicosanol (14.6%), Octacosane (5.4%), and γ Amorphen (5.2%). In vitro antioxidant activities of prepared essential oils were determined by using ABTS and DPPH methods and compared to those of the Trolox. Among the tested samples, flowers had the highest phenolic content with 22.02 mg equivalents Catechin/g of extract and roots presented the lowest phenolic content with 3.12 mg equivalents Catechin/g of extract. To evaluate in vitroantifungal activity, all volatile oils were tested against four fungi (two dermatophytes and two hyphamycets).

 

Keys words: Cotula coronopifolia L, essential oils, chemical composition, by gas chromatography/ mass spectrum (GC/MS), antioxidant activity, antifungal activity.