May 2012
Molecular cloning and phylogenetic analysis of the E gene of transmissible gastroenteritis virus (TGEV) isolated in China
Porcine transmissible gastroenteritis caused by transmissible gastroenteritis virus (TGEV)induces acute, high-contact intestinal infectious diseases in pigs. TGEV encodes four structural proteins including spike (S), membrane (M), envelope (E) and nucleoprotein (N), of these, the genes of S, M and N respectively had been deeply elucidated. However, the real function of E protein was still...
May 2012
Genotypic variability for tolerance to salinity and phosphorus deficiency among N2-dependent recombinant inbred lines of Common Bean (Phaseolus vulgaris)
Common bean (Phaseolus vulgaris L.) is often subject to various environmental constraints including soil salinity and phosphorus deficiency as major limitations for the yield of most grain legumes, especially when the plant growth depends upon N2 fixation. In order to assess the genetic variation for tolerance to moderate salinity and phosphorus deficiency and identify the related morphological, physiological...
May 2012
Optimization, production and purification of cellulase enzyme from marine Aspergillus flavus
Water samples were collected from five different places at Muthupettai mangrove along the east cost of Tamil Nadu, India. Five different Aspergillus species were isolated and identified by using lacto phenol cotton blue staining method. From this, Aspergillus flavuswas tested in Modified Park’s Agar for its cellulase enzyme production in different physico-chemical properties of substrates. The...
May 2012
Comparative analysis of endophytic mycobiota in different tissues of medicinal plants
Water samples were collected from five different places at Muthupettai mangrove along the east cost of Tamil Nadu, India. Five different Aspergillus species were isolated and identified by using lacto phenol cotton blue staining method. From this, Aspergillus flavuswas tested in Modified Park’s Agar for its cellulase enzyme production in different physico-chemical properties of substrates. The...
May 2012
The stability of micronutrients in fortified food stuffs after processing and storage: Iodine in salt and iron in wheat flour
Salt was fortified by potassium iodate in the level 66 mg/kg of salt with wet method of fortification. Then the iodized salt was stored using three packaging materials; low density polyethylene (LDPE), high density polyethylene (HDPE) and woven high density polyethylene (WHDPE) at two conditions: room temperature (20-25°C) and medium RH (50-60%) for about 6 months and accelerated temperature (40°C) and high...
May 2012
Temporal and spatial variations of phytoplankton in the Caspian Sea
Geographic information system (GIS) can be used to perform a number of fundamental spatial analysis operations. Its major advantage is that it allows the user to identify the spatial relationships between various map features. In this study, phytoplankton cell abundance, biomass and species composition were evaluated in the Iranian coasts of the Caspian Sea during 1996 to 1997 and 2005 to 2006. Totally 1562 water...
May 2012
NMR Shielding and thermodynamic density functional theory (DFT) studies of solvent effect on B10N11H7(Ala)2 Nano Cone
B3LYP/3-21G density functional theory (DFT) are carried out on the structure and stability of B10N11H7(Ala)2 in bulk and different solvent medium, using Gaussian 98 package of program. NMR parameters and thermodynamic properties were calculated to obtain stability and solvent effect. It was found that computationally efficient solvent modeling is possible to find better results and also molecular structure of...
May 2012
Effect of Ultraviolet-C (UV-C) irradiation on the virulence genes expression in Vibrio parahaemolyticus and Vibrio alginolyticus
In this study, Vibrio parahaemolyticus and Vibrio alginolyticus, marine foodborne pathogens, were treated with Ultraviolet-C (UV-C) irradiation (240 J.m-2) to evaluate alterations in their virulence genes expression levels. Firstly, we searched for the presence of eight Vibrio cholerae virulence genes, toxR, toxS, toxRS, ctxA, zot, ace, toxT, and virulence...
May 2012
Characterization of Vibrio alginolyticus and Vibrio parahaemolyticus isolated from Penaeus monodon: Antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis
Vibrio alginolyticus and Vibrio parahaemolyticus are important pathogens which cause serious episode to shrimp. We characterize the suitability of randomly amplified polymorphic DNA (RAPD), and plasmid profiling for rapid molecular characterization of pathogenic bacteria for epidemiological and antibiogram study to find out the relationship between plasmids and bacterial sensitivity to...
May 2012
Seasonal examination of phytoplankton abundance and assemblages in Myall Lake and Bombah Broadwater, New South Wales, Australia
The Myall Lakes are a series of interconnected, shallow and poorly flushed coastal lakes located within the Myall Lakes National Park in NSW, Australia. The following field study aims to uncover patterns in temporal variability in water quality and phytoplankton abundance, diversity and assemblage structure, in the upper and lower water bodies of the Myall Lakes system. The result showed that the two lakes differ...
May 2012
Xylanase production under solid state and submerged fermentation conditions by bacterial strains
Bacterial strains were isolated from soil samples collected at different parts of Western Ghats of India. The strains were screened for xylanase production using xylan-red agar medium. On the basis of clearing zones formed, twelve bacterial strains were selected and identified on the basis of 16S rRNA sequence. Solid state and submerged fermentations were done to identify strains that could produce maximum amount of...
May 2012
Long-term outcomes of chronic hepatitis C patients treated with pegylated interferon 2a plus ribavirin in Iran
Combination of interferon alfa and ribavirin is the first therapeutic choice in chronic hepatitis C. The aim of this study is to assess long-term outcome of chronic hepatitis C patients treated with pegasys plus ribavirin. We evaluated a cohort of 49 chronic hepatitis C patients who were treated for 48 weeks with pegylated interferon alpha 2a and ribavirin and followed up till week 72 in order to evaluate sustained...
May 2012
The effects of solid wood waste discharge on the physico-chemical and microbial characteristics of the Warri river
Water obtained from three locations from September to November, 2010 at the point of discharge of the sawmill wastes into the Warri river (Point 2) and at 100 m before (Point 1) and after (Point 3) the discharge were analyzed using standard methods to determine the effects of the wastes on the physico-chemical and microbial parameters of the River. While mean values for temperature, alkalinity, sulphate, carbonate,...
May 2012
Evaluation of the reaction of durum wheat genotypes (Triticum durum Desf.) to drought conditions using various stress tolerance indices
In this study, 40 genotypes of durum wheat (Triticum durum) originating from Iran and Azerbaijan Republic were evaluated in both water-stressed and well-watered environments in 3 years 2008 to 2011 cropping years. In each environment, the genotypes were evaluated using a randomized complete block design with three replications. From the grain yield data, drought tolerance indices comprising of stability tolerance index...
May 2012
Seasonal influence on bioactivity of seaweeds against plant pathogenic bacteria Xanthomonas axonopodis pv. citri (Hasse) Vauterin et al.
In the present study, thin-layer chromatography (TLC) method was adopted for the fractionation of antibacterial substances from the crude extracts obtained in 1:1(v/v) chloroform : methanol of red seaweeds comprising Portieria hornemannii (Lyngbye) P. Silva, Gracilaria crassa (Harvey) J. Ag., brown Dictyota bartyrensiana Lamouroux,Hydroclathrus clathratus (C.Agardh)...
May 2012
Pathogenicity of recent field isolate of Avian Adenovirus Serotype-IV of hydropericardium syndrome (Angara disease)
The pathogenicity of recent field isolate of Avian Adenovirus Serotype IV (AAS-IV) was determined during the present study. During the present investigations, Hydropericardium Hepatitis Syndrome (HHS) showed reduced pathogenicity as compared to the isolates studied on the inception of the syndrome in 1987-1988. Pathogenicity of the virus was determined through LD50 and challenge protection test. The LD50 titre...
May 2012
Antibiogram types of Klebsiella aerogenes isolated from urinary tract infection (UTI)
Thirty three (33) strains of Klebsiella aerogenes isolated from 197 in-and out-patients with symptoms of urinary tract infection (UTI) at University of Benin Teaching Hospital (UBTH), Benin City were used for this study. Twenty-one antibiotics were used for the antibiogram typing. Organisms were isolated from 65 positive samples, with the most prevalent beingStaphylococcus aureus (40.0%), others...
May 2012
Effect of row spacing and sowing date on yield and yield components of common bean (Phaseolus vulgaris L.)
Seed legumes are extremely important crop plants, which are widely cultivated in the Middle East. The study was conducted to determine the effect of different seeding times and seed rates on the growth, yield and quality of common bean (Phaseolus vulgaris L.). The experiment was laid out using RCBD under split plot arrangement with three replications. Three Row space as main plot R1 (50), R2 (40) and...
May 2012
Retracted: General steps in the production and establishment of transgenic chicken lines carrying a bacterial lacZ gene expressing β-galactosidase
Replication-defective retroviral vectors are efficient vehicles for the delivery of exogenous genes, and they may be used in the generation of transgenic animals. The replication-defective retroviral SNTZ vector carrying the lacZ gene with a nuclear localized signal was injected into the subgerminal cavity of freshly laid eggs. Subsequently, the eggs were allowed to hatch, and the chickens were screened for...
May 2012
Biofilm production, adherence and hydrophobicity of starved Shigella in seawater
In this work, we incubated four Shigella strains for one month at room temperature and at 4°C. Their adhesion capacity to KB cells, biofilm formation on microplate and surface hydrophobicity of starved cells were investigated. Our results showed that the stressed cells exhibited a remarkable increase in their hydrophobicity from 19 to 34% and in their capacity to produce biofilm. Adherence assays to KB...
May 2012
Evaluation of in vitro antiviral activity of Chelidonium majus L. against herpes simplex virus type-1
Chelidonium majus Linnaeus (L) has been used in a variety of practical applications in medical sciences. Its antiviral activity against herpes simplex virus type-1 (HSV-1) was investigated in cell culture. C. majus L. extract was found to be non-toxic to Vero cells (African green monkey kidney cells) up to concentration 1000 mg/ml and inhibited the growth and development of HSV-1 in dose-dependent...
May 2012
Production and optimization of D-amino acid oxidase which is involved in the biosynthesis of β-lactam antibiotics
A total of sixty seven fungal isolates representing 40 fungal species related to 14 genera were screened for their abilities to produce endocellular D-amino acid oxidase enzyme. The most active fungal isolates were Fusarium heterosporum and Nectria haematococca, producing 210.41 and 207.94 units/ml, respectively. Maximum activity of D-amino acid oxidase produced by F....
May 2012
Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1
Chitinase was isolated and purified from Gliocladium catenulatum through ammonium sulfate sedimentation, sephadex G-25 gel filtration, deionization, ultra filtration condensation, negative ion exchange separation, and non-denaturing gel electrophoresis. A 51 kDa chitinase was purified from the fungus through sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum temperature and pH for...
May 2012
Seroprevalence of leptospiral infection in horses, donkeys and mules in East Azerbaijan province
This study was conducted on 490 animals (390 horses, 80 donkeys and 20 mules) in East Azarbaijan province in Iran in order to verify seroprevalence of leptospiral infection in 2011. Sera were initially screened at dilution of 1:100 against 6 live serovars of Leptospira interrogans: Pomona, Canicola, Hardjo, Ballum, Icterohaemorrhagiae and Grippotyphosa using the microscopic agglutination test. The prevalence of...
May 2012
Chemical composition, in vitro antifungal and antioxidant activities of essential oil from Cotula coronopifolia L. growing in Tunisia
The chemical composition of Cotula coronopifolia L. essential oils obtained from the aerial parts (flowers, leaves, stems) and roots by hydro distillation was analyzed by gas chromatography/ mass spectrum (GC/MS). Fifty seven components were identified. The most important compound detected in leaves was Agarospirol (10.43%). Hexacosane was identified in flowers with an abundance percentage of (31.7%) and...
May 2012
Antimicrobial activity of a biosurfactant produced by Bacillus licheniformis strain M104 grown on whey
The aim of this study is to investigate the antimicrobial effect of the lipopeptide biosurfactant produced by Bacillus licheniformis strain M104 grown on whey. The biosurfactant was investigated for potential antimicrobial activity by using disc-diffusion method against several Gram positive bacteria (Bacillus subtilis, Bacillus thuringiensis (2 strains), Bacillus cereus, Staphylococcus...
May 2012
Comparative inhibitory effect of xylitol and erythritol on the growth and biofilm formation of oral Streptococci
Our aims were to examine the effects of xylitol and a novel polyol sweetener, erythritol, on growth of oral Streptococci and compare their effects. The inhibitory effects of xylitol and erythritol on Streptococcus strains, as well as on streptococcal biofilm formation were examined. Streptococcus mutans, Streptococcus sobrinus, and Streptococcus sanguiniswere used as...
May 2012
Cyanobacterial abundance and diversity in coastal wetlands of Kanyakumari District, Tamil Nadu (India)
Coastal wetlands are physiologically unique from freshwater and marine water environments. They support the growth of both marine and freshwater organisms with affluent diversity. In this study, abundance and diversity of cyanobacteria from the coastal wetlands of Kanyakumari District (Tamil Nadu) in India was studied. Water samples were collected from ten different sampling sites during the month of January 2010 to...
May 2012
Production, optimization and purification of lipase from Pseudomonas aeruginosa
Microbial lipases today occupy a place of prominence among biocatalysts owing to their ability to catalyze a wide variety of reactions. Out of 14 bacterial isolates obtained from wastewater sample. The isolates were grown on a selective medium agar that contained tween 80 or olive oil as the only source of carbon for detection of lipase activity 6 exhibited lipase activity. Pseudomonas aeruginosa (Ps5) was...
May 2012
Microorganisms associated with computer keyboards and mice in a university environment
A study was carried out to isolate and to identify microorganisms associated with computer keyboards and mice in computer centers and cyber café located in Kogi State University, Anyigba, Nigeria and its environs. Samples were collected from five different cyber café and computer centers. The samples were collected from three computer keyboards and mice in each cyber café and computer centers. The...
Advertisement
Advertisement
